Method for synthesizing nano-preparations for visual guidance and combined immunotherapy of tumors by Gd:CuS mineralized Sendai virus

A Sendai virus and immunotherapy technology, applied in the field of visualization of Gd:CuS mineralized Sendai virus to guide the synthesis of tumor combined immunotherapy nano preparations, can solve the problems of harsh environment, pollution, cumbersome process, etc., to achieve no side effects, Excellent biocompatibility and high photothermal conversion efficiency

Pending Publication Date: 2019-09-06
TIANJIN UNIV
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  • Abstract
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  • Claims
  • Application Information

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It solves the problem that the traditional synthesis method is too

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  • Method for synthesizing nano-preparations for visual guidance and combined immunotherapy of tumors by Gd:CuS mineralized Sendai virus

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Experimental program
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Embodiment 1

[0020] The synthesis method of SeV@Gd:CuS tumor combined immunotherapy nano-preparation, the specific steps are as follows:

[0021] (1) Weigh 4.25 mg of copper chloride dihydrate (CuCl 2 2H 2 O) powder, ultrasonically dissolved after adding 5mL of ultrapure water, to obtain CuCl with a concentration of 0.005M 2 2H 2 O aqueous solution.

[0022] (2) Weigh 9.3 mg of gadolinium chloride hexahydrate powder (GdCl 3 ·6H 2 O), ultrasonically dissolved after adding 1mL ultrapure water, the concentration of GdCl obtained was 0.025M 3 ·6H 2 O aqueous solution.

[0023] (3) One-pot method for synthesizing SeV@Gd:CuS nanoparticles: place a single-mouth bottle containing 2.5ml of Sendai virus solution with a concentration of 0.005mg / ml on a magnetic stirrer in a water bath at 37°C, and then stir Under the condition, use a disposable dropper to drop the CuCl in the above configuration 2 2H 2 O and GdCl 3 ·6H 2 O solution, after dropwise addition, continue to stir for three minu...

Embodiment 2

[0028] The synthesis method of SeV@Gd:CuS tumor combined immunotherapy nano-preparation, the specific steps are as follows:

[0029] (1) Weigh 8.5 mg of copper chloride dihydrate (CuCl 2 2H 2 O) powder, ultrasonically dissolved after adding 5mL of ultrapure water, to obtain CuCl with a concentration of 0.01M 2 2H 2 O aqueous solution.

[0030] (2) Weigh 18.6 mg of gadolinium chloride hexahydrate powder (GdCl 3 ·6H 2 O), ultrasonically dissolved after adding 1mL of ultrapure water to obtain GdCl with a concentration of 0.05M 3 ·6H 2 O aqueous solution.

[0031] (3) A one-pot method for synthesizing SeV@Gd:CuS nanoparticles: put a one-mouth bottle containing 5ml of Sendai virus solution with a concentration of 0.01mg / ml on a magnetic stirrer in a water bath at 37°C, and then Next, use a disposable dropper to drop the CuCl in the above configuration 2 2H 2 O and GdCl 3 ·6H 2 O solution, after dropwise addition, continue to stir for three minutes;

[0032] (4) Configu...

Embodiment 3

[0036] The synthesis method of SeV@Gd:CuS nano-diagnostic agent, the specific steps are as follows:

[0037] (1) Weigh 17mg of copper chloride dihydrate (CuCl 2 2H 2O) powder, ultrasonically dissolved after adding 5mL ultrapure water, to obtain CuCl with a concentration of 0.02M 2 2H 2 O aqueous solution.

[0038] (2) Weigh 37.2 mg of gadolinium chloride hexahydrate powder (GdCl 3 ·6H 2 O), ultrasonically dissolved after adding 1mL ultrapure water, to obtain GdCl with a concentration of 0.1M 3 ·6H 2 O aqueous solution.

[0039] (3) One-pot method for synthesizing SeV@Gd:CuS nanoparticles: put a one-mouth bottle containing 10ml of Sendai virus solution with a concentration of 0.02mg / ml on a magnetic stirrer in a water bath at 37°C, and then Next, use a disposable dropper to drop the CuCl in the above configuration 2 2H 2 O and GdCl 3 ·6H 2 O solution, after dropwise addition, continue to stir for three minutes;

[0040] (4) Configure a sodium hydroxide (NaOH) solut...

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Abstract

The invention discloses a method for successfully synthesizing nano-preparations for combined therapy of tumors by strategy biomimetic simulation of Gd:CuS mineralized Sendai virus (SeV@Gd:CuS). The method includes the steps: 1) preparation of a CuCl2.2HO2 aqueous solution; 2) preparation of a GdCl3.6H2O aqueous solution; and 3) one-pot synthesis of SeV@Gd:CuS nanoparticles. Sendai virus has a complex structure, can systemically activate the immune function of a body and causes all-round killing and clearance of tumors. The SeV@Gd:CuS nanoparticles have significant T1-weighted magnetic resonance/photoacoustic dual-mode imaging ability, have high photothermal conversion efficiency under near-infrared laser irradiation, can highly efficiently guide the follow-up photothermal therapy of tumors, have excellent biocompatibility and do not generate side effects.

Description

technical field [0001] The present invention relates to a biomimetic simulation of a strategy for the mineralization of Sendai virus (SeV@Gd:CuS) via Gd:CuS to successfully synthesize a visualization-guided Background technique [0002] At present, tumor is one of the most threatening diseases to human beings in the world. Immunotherapy can treat cancer by activating the human immune system and relying on its own immune function to kill cancer cells and tumor tissues. Therefore, immunotherapy is one of the most effective ways to treat tumors. In view of the complex structure of Sendai virus, which contains nucleocapsid protein, phosphoprotein, matrix protein, glycoprotein, fusion protein, envelope, hemagglutinin, neuraminidase and ss-RNA and other components, as a foreign body, it can stimulate The systemic immune system produces a cytokine storm (such as interferon, tumor necrosis factor and various interleukins, etc.), and makes dendritic cells (DC cells, presenting tumo...

Claims

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Application Information

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IPC IPC(8): A61K35/768A61K41/00A61P35/00
CPCA61K35/768A61K41/0052A61P35/00A61K2300/00
Inventor 郑斌明东王树超徐亚楠
Owner TIANJIN UNIV
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