Method for sampling and counting cells cultured on microcarriers

Abstract

The invention discloses a method for sampling and counting cells cultured on microcarriers. The method for sampling the cells cultured on the microcarriers comprises the steps that (A1) a certain weight of the microcarriers used for cell culture are weighed and placed into a sampling tube, liquid is added for simulating a cell culture process, soaking is carried out for 0-24 hours at 4-60 DEG C, the scale corresponding to the volume occupied by the microcarrier calibrated on the sampling tube serves as a standard scale; (A2) the cells cultured on the microcarriers are sampled as follows: the sampling tube is used for sampling the microcarriers from a cell culture system, so that the volume of the microcarriers reaches the standard scale. Compared with a traditional suspension quantitativesampling method, the repeatability of three samples by utilizing a provided microcarrier quantitative sampling method to count the cells in the microcarrier cell culture system is better (the varianceis smaller), and the count is very close to the actual total number.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a method for sampling and counting cells cultured on microcarriers. Background technique [0002] The large-scale industrial culture technology of animal cells is very important for biological industries such as biopharmaceuticals, vaccine production, and cell therapy. The traditional two-dimensional cell culture method has many inconvenient factors, such as large space required, low yield, and difficult control, etc., which restricts the large-scale cultivation of animal cells. The emergence of microcarrier cell culture technology makes the process of industrial production of animal cells easier, and it is currently recognized as the most promising culture model. Although this emerging cell culture technology is a popular application technology in the fields of medicine and pharmaceuticals in the future, there are still some aspects that need to be optimized and improved. Among the...

AI Technical Summary

Problems solved by technology

This sampling process requires extremely high uniformity, and the process and method of each sampling should be kept stable. However, because the density of microcarriers is much larger than that of the medium, sedimentation is prone to occur, and it is difficult to ensure the microcarriers in the actual sampling process. Evenly suspended in the culture medium, so although the same volume of suspension is also drawn, the content of microcarriers in it will be deviated due to the fact that the original solution is not a homogeneous suspension

In the cell industry, large-volume bioreactors and other instruments are generally used to culture cells on a large scale. This kind of culture process is not easy to sample evenly, which leads to cell counting and conversion of the number of cells in the entire culture system according to the volume of the suspension. There is a large error with the real number of cells, resulting in inaccurate cell counts and unable to accurately measure the growth of cells

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