Kit for detecting genetic susceptibility of gestational diabetes mellitus

A kit and susceptibility technology, applied in the fields of molecular biology and medicine, can solve problems such as the inability to take preventive measures for GDM in advance, the lack of kits, and the low sensitivity of genetic susceptibility

Pending Publication Date: 2019-09-13
武汉正轩宇生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although there are studies on the risk association between polymorphic sites of TCF7L2 gene and MTNR1B gene and GDM in the prior art, they are only conducted on polymorphic sites of a single gene, which are used to detect GDM separately. The sensitivity of genetic susceptibility is not high, and there is a lack of kits that can be used to detect genetic susceptibility to GDM in the prior art, and it is impossible to screen out high-risk groups of GDM susceptibility in time, so it is impossible to take preventive measures for GDM in advance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Through the genotyping of 335 GDM patients and 410 normal pregnant women, the frequency of the haplotype composed of the SNP site rs290487 on the TCF7L2 gene and the SNP site rs10830963 on the MTNR1B gene was detected in the case group and the normal group , it was found that individuals who carry both the C allele at rs290487 of TCF7L2 and the G allele at rs10830963 of MTNR1B gene have a 2-3 times increased risk of developing GDM (that is, when two gene loci have mutations related to GDM at the same time, the highest risk of disease).

Embodiment 2

[0023] The steps of using the kit for detecting genetic susceptibility to gestational diabetes provided by the present invention are as follows:

[0024] Step 1: Extraction of DNA template

[0025] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption method.

[0026] Step 2: Real-time quantitative PCR reaction

[0027] Use the fluorescent quantitative PCR set in the detection kit, which contains the following primer pairs and fluorescent probe pairs:

[0028] Sense primer 1: 5'-GTGAGTCTTCCAACCCAGTACAAA-3' (SEQ ID NO: 1)

[0029] Antisense primer 1: 5'-TGGATCAAACACCTTTTCTCATTTTC-3' (SEQ ID NO: 2)

[0030] Sense primer 2: 5'-GCCCCCAGTGATGCTAAGAA-3' (SEQ ID NO: 3)

[0031] Antisense primer 2: 5'-GGCAGAATATTCCCATCAGGAA-3' (SEQ ID NO: 4)

[0032] Fluorescent gene probe 1 with VIC: TGACACCATGCAAAA (SEQ ID NO: 5)

[0033] Probe 1 with FAM fluorophore: CACCACGCAAAAT (SEQ ID NO: 6)

[0034] Fluorescent gene probe 2 with VIC: TCACACCATCTGCT (SEQ ID NO: 7)

...

Embodiment 3

[0043] Services for testing pregnant women for genetic susceptibility to gestational diabetes

[0044] Step 1: Extraction of DNA template

[0045] The physicians in the laboratory department of the hospital will guide the subjects to use oral swabs to sample oral epithelial cells, and use the silica gel adsorption method to extract DNA from oral epithelial cells (blood samples and other biological samples can also be used).

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Abstract

The invention relates to a kit for detecting genetic susceptibility of gestational diabetes mellitus. The kit comprises a specific primer pair and a specific fluorescent probe pair which simultaneously detect the rs290487 SNP locus on TCF7L2 gene and the rs10830963 SNP locus on MTNR1B gene, Taq enzyme, dNTP mixed solution, an MgCl2 solution, a fluorescent quantitative PCR reaction buffer solutionand deionized water. The invention has the beneficial effects that: the genotypes of the rs290487 SNP locus on the TCF7L2 gene and the rs10830963 SNP locus on the MTNR1B gene are simultaneously detected for the first time, and the polymorphism of the two loci and dietary factors have interaction. Comprehensive evaluation indicates that the incidence risk of the gestational diabetes can be effectively predicted, and therefore accurate nutrition intervention or other treatment measures can be carried out on high-risk groups of the gestational diabetes before pregnancy as soon as possible.

Description

technical field [0001] The invention belongs to the fields of molecular biology and medicine, and in particular relates to a kit for detecting genetic susceptibility of gestational diabetes. Background technique [0002] Gestational diabetes mellitus (GDM) is abnormal glucose tolerance that occurs or is discovered for the first time during pregnancy and is one of the most common pregnancy complications. The incidence of gestational diabetes worldwide is increasing year by year with the increase in the incidence of overweight and obesity. In 2018, the prevalence of gestational diabetes in Chinese pregnant women was reported as high as 14.8%. With the change of my country's birth policy and the increase of advanced maternal age, the risk of gestational diabetes in pregnant women in my country will further increase. Abnormal blood sugar during pregnancy will increase the risk of pregnancy complications such as gestational hypertension, preeclampsia, premature delivery, and po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/156
Inventor 杨雪锋李静吴欣江吴远珏
Owner 武汉正轩宇生物科技有限公司
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