Method for quantitatively determining content of AGEs components in milk powder by adopting HPLC-MS/MS method

A quantitative measurement, milk powder technology, applied in the direction of measuring devices, material separation, analysis of materials, etc., can solve the problems of lack of effective research methods and technical means

Inactive Publication Date: 2019-09-17
NANJING INST OF PROD QUALITY INSPECTION
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] How to identify potentially harmful AGEs components in food and the quality control

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quantitatively determining content of AGEs components in milk powder by adopting HPLC-MS/MS method
  • Method for quantitatively determining content of AGEs components in milk powder by adopting HPLC-MS/MS method
  • Method for quantitatively determining content of AGEs components in milk powder by adopting HPLC-MS/MS method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] Through the screening of the cell membrane solid-phase system, milk powder mainly contained three AGEs components: carboxymethyllysine (CML), carboxyethyllysine (CEL) and pyrrolidine (Pyr). This section adopts HPLC-MS / MS analysis method, electrospray ion source multiple reaction monitoring (MRM) mode, through methodological demonstration, in order to construct a quantitative analysis method for the simultaneous determination of CML, CEL and Pyr in milk powder, which is the monitoring of AGEs in milk powder and control provide the basis.

[0021] 1 Experimental materials and instruments

[0022] (1) Reagents and materials: n-hexane (Sinopharm Chemical Reagent Co., Ltd., batch number: 20160712); ethanol (Nanjing Chemical Reagent Co., Ltd.); double-circle qualitative filter paper (General Electric Biotechnology Hangzhou Co., Ltd.); sodium hydroxide ( Nanjing Chemical Reagent Co., Ltd., batch number: 170608244E); hydrochloric acid (Shanghai Lingfeng Chemical Reagent Co., L...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Mobile phase aaaaaaaaaaa
Mobile phase aaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the field of food detection, and relates to a method for quantitatively determining the content of AGEs (advanced age test) components in milk powder by adopting an HPLC-MS/MS (high performance liquid chromatography-mass spectrometry/mass spectrometry) method. The detection conditions of the method comprise: chromatographic conditions: C18 column; a sample injection amount of 10 mu L; a flow rate of 0.5 mL/min; column temperature: 30 DEG C; gradient elution which is carried out with mobile phase A of high purity water containing 5 mM trifluoroacetic acid and mobile phase B of acetonitrile containing 5 mM trifluoroacetic acid; mass spectrum conditions: electrospray ion sources; positive ion scan mode; multiple reaction monitoring mode; residence time 500 ms; ion source temperature: 200 DEG C; desolventizing temperature: 380 DEG C; desolventizing gas flow rate: 500L/h; an airflow rate of a taper hole of 50L/h; a voltage of the sample introduction cone of 20V; and capillary voltage: 3 kV, wherein the desolventizing gas and the taper hole gas are nitrogen, and the collision gas is argon. The method is simple and convenient to operate, rapid, high in accuracy and good in sensitivity.

Description

[0001] Technical field: [0002] The invention belongs to the field of food detection, and in particular relates to a method for quantitatively determining the content of AGEs in milk powder by using HPLC-MS / MS method [0003] Background technique: [0004] Some chemical changes that occur during food processing and storage, while changing the structure and function of food components, also produce some harmful substances that cannot be ignored, namely advanced glycation end products (AGEs), which are produced by Maillard It is a kind of stable product formed by reaction, fat oxidation reaction or polyol degradation, which can be seen in various foods such as dairy products, fried food fried dough sticks, twists, French fries in foreign fast food, fried bread and French chips in snacks , fried biscuits, etc. The formation of AGEs is closely related to the raw materials, processing technology, processing temperature, processing time and storage conditions of food. A large amou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/72G01N30/34G01N30/04G01N30/06G01N30/14G01N30/86
CPCG01N30/02G01N30/04G01N30/06G01N30/14G01N30/34G01N30/72G01N30/8634G01N2030/042G01N2030/062G01N2030/067G01N2030/146
Inventor 朱毛毛胡飞杰顾慧丹楼超群王晓丽
Owner NANJING INST OF PROD QUALITY INSPECTION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap