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Application of SERPINGI protein used as marker in development of reagent for active tuberculosis diagnosis

A tuberculosis, active technology used in the field of medical immunology diagnosis

Pending Publication Date: 2019-09-17
中国人民解放军总医院第八医学中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently no SERPING1 protein is used to diagnose active pulmonary tuberculosis

Method used

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  • Application of SERPINGI protein used as marker in development of reagent for active tuberculosis diagnosis
  • Application of SERPINGI protein used as marker in development of reagent for active tuberculosis diagnosis
  • Application of SERPINGI protein used as marker in development of reagent for active tuberculosis diagnosis

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Experimental program
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Effect test

Embodiment 1

[0076] Embodiment 1, the application of the relative expression of SERPING1 gene in the diagnosis of active tuberculosis

[0077] 1. Obtaining of peripheral blood samples

[0078] 1. Distinguish peripheral blood samples from patients with latent tuberculosis infection from those from healthy people

[0079] Latent tuberculosis infection patients and healthy people have no signs or symptoms of tuberculosis, and they can be distinguished according to the following steps:

[0080] a. Coated

[0081] (1) Take a 96-well plate, add 100 μL of IFN-γ monoclonal capture antibody to each well, and coat overnight at 4°C.

[0082] (2) After completing step (1), take the 96-well plate, discard the liquid phase, add pH 7.4, 0.01M PBS buffer solution to wash twice (1 min each time), and pat dry.

[0083] (3) After completing step (2), the 96-well plate was taken, and 200 μL of 0.01 M PBS buffer at pH 7.4 containing 2% (v / v) BSA was added to each well, and incubated at 37° C. for 1 h.

[0...

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Abstract

The invention discloses application of SERPINGI protein used as a marker in development of a reagent for active tuberculosis diagnosis. Compared with a healthy person and a latent tuberculosis infection person, the expression quantity of an SERPINGI gene in PBMCs of an active tuberculosis patient is remarkably increased, and the expression quantity of the SERPINGI can be used for distinguishing the active tuberculosis patient and the latent tuberculosis infection person and can be used for distinguishing the active tuberculosis patient and the healthy person. Therefore, the SERPINGI protein and / or SERPINGI gene can be used as the marker in development of the reagent for the active tuberculosis diagnosis. The SERPINGI protein has an important application value.

Description

technical field [0001] The invention belongs to the technical field of medical immunology diagnosis, and in particular relates to the application of SERPING1 protein as a marker in developing reagents for diagnosing active tuberculosis. Background technique [0002] Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis (MTB), which is mainly transmitted through the respiratory tract. After MTB infects the human body, three different results can occur. One is that the body’s immunity is better, and MTB is directly cleared; the other is that MTB is suppressed by the body’s immune system, but cannot be completely cleared, and develops into latent tuberculosis infection (LTBI). ; The third is that MTB proliferates rapidly in the body and develops into active tuberculosis. Tuberculosis is a major infectious disease that my country needs to focus on prevention and control. [0003] At present, the diagnosis of tuberculosis mainly includes imaging diagnosis, ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/543G01N33/531G01N33/68
CPCG01N33/577G01N33/543G01N33/531G01N33/68
Inventor 杨秉芬翟斐蒋静安红娟苏瑾文王若曹志红刘艳华
Owner 中国人民解放军总医院第八医学中心
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