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Eye mask nutrient layer containing Periplaneta americana extract and preparation method thereof

The technology of Periplaneta americana and its extract is applied to the nutritive layer of eye mask containing Periplaneta americana extract and its preparation field, which can solve the problems of polypeptide denaturation and activity reduction, and achieve the purpose of promoting secretion, relieving dry eye symptoms and improving tear secretion. insufficient effect

Active Publication Date: 2021-11-12
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the active ingredients in the extract of Periplaneta americana are mostly proteins, polypeptides, and amino acids that are sensitive to stable temperature. The traditional drying and drying process will lead to denaturation of polypeptides and reduce their activity.

Method used

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  • Eye mask nutrient layer containing Periplaneta americana extract and preparation method thereof
  • Eye mask nutrient layer containing Periplaneta americana extract and preparation method thereof
  • Eye mask nutrient layer containing Periplaneta americana extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0028] Example 1: Separation, purification and identification of anti-inflammatory active peptides in Periplaneta americana

[0029] Material:

[0030] Dried body of Periplaneta americana.

[0031] instrument:

[0032] ALPHA 1-2LDplus freeze dryer (Christ, Germany), matrix-assisted laser desorption ionization / tandem time-of-flight mass spectrometer TOF / TOF 5800System (AB SCIEX, USA). Chromatographic column: dextran gel chromatography column SephadexG-75; amino column Luna NH 2 100A (Phenomenex, USA); Lichrospher C 18 Column (Agilent, USA).

[0033] Reagent:

[0034] Protease inhibitor PMSF (Soleibao Company), aprotinin Aprotinin melanization inhibitor phenylthiourea (US Sigma Company); acetonitrile CAN (Fisher Company); trifluoroacetic acid TFA (Aladdin Company). Other reagents were of domestic analytical grade.

[0035] Test method and conditions:

[0036] The dried worm body of Periplaneta americana was pulverized and extracted three times with hot water under reflu...

example 2

[0045] Example 2: Preparation of eye mask nutrient layer

[0046] Material:

[0047] American cockroach extract (provided by Yunnan Tengyao Pharmaceutical Co., Ltd.)

[0048] purified water

[0049] Non-woven fabric substrate (plant viscose fiber 40%, pure cotton fiber 10%, wood pulp fiber 20%, polyester fiber 30%, the gram weight of described non-woven fabric is 40g / m 2 . )

[0050] Other accessories

[0051] instrument:

[0052] Decompression soaking medicine drying equipment

[0053] vacuum pump

[0054] Slitter

[0055] Hot compress steam eye mask forming packaging equipment

[0056] Specifically, the preparation of the nutrient layer in the steam eye mask of the present invention comprises the following steps:

[0057] 1) Dispensing: Mix the extract of Periplaneta americana with purified water at a ratio of 1.5:1000, stir evenly in a dispensing tank, and let it stand at room temperature for 2 hours until the air bubbles are completely eliminated.

[0058] 2) So...

example 3

[0062] Example 3: Comparison of content determination of active markers of Periplaneta americana by different preparation methods.

[0063] Material:

[0064] Sample A: Natural shade drying style, take Example 1, after the preparation and soaking process, the non-woven fabric substrate that has not been dried under reduced pressure. , standard atmospheric pressure, RH20~30%,) fully and naturally dry in the shade to obtain test samples.

[0065] Sample B: a test sample dried under reduced pressure, a small roll of non-woven fabric substrate prepared according to Example 1.

[0066] Sample C: High-temperature drying sample. Take the non-woven fabric substrate that has not been dried under reduced pressure after the preparation and soaking process of Example 1. Under standard atmospheric pressure, it is dried at 105°C for 60 minutes at high temperature to obtain a test sample. c.

[0067] Instrument: High Performance Liquid Chromatography

[0068] Taking the active marker con...

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Abstract

The present invention provides an eye mask nutrient layer containing Periplaneta americana extract and a preparation method thereof, which comprises a non-woven fabric substrate and tripeptide-1, palmitoyl tripeptide-1, palmitoyl tripeptide-5, acetyl di Five activity markers of peptide-1 and palmitoyl pentapeptide-4. After the extract of Periplaneta americana is dried under reduced pressure, a variety of active markers are stably attached to the non-woven fabric substrate, and it can effectively ensure that the active peptide components do not undergo a large amount of denaturation during the preparation process.

Description

technical field [0001] The invention relates to an eye mask containing biological peptides, in particular to an eye mask nutrient layer containing a Periplaneta americana extract and a preparation method thereof. Background technique [0002] With the popularization of various video terminals, the incidence of visual fatigue is rising rapidly. A large number of studies have shown that using video terminals for a long time can induce dry eye syndrome, which has a certain degree of impact on people's quality of life and work. Chinese medicine hot ironing is to soak gauze with decocted medicinal liquid and apply it to the eyes while it is hot. It is a common treatment method commonly used in ophthalmology of traditional Chinese medicine to relieve dry eye syndrome. The hot compress steam eye masks currently on the market use the self-heating principle of the iron powder oxidation process to provide continuous hot compress for 20 to 30 minutes (average temperature 40±5°C). Som...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F9/04A61K35/64A61P27/02
CPCA61F9/045A61K35/64A61P27/02
Inventor 李玥胡竹林吕乐春陈真许洪陆地
Owner KUNMING MEDICAL UNIVERSITY
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