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Application of Aurora-B kinase and Survivin in preparation of colorectal cancer diagnosis kit

A diagnostic kit, technology for colorectal cancer, applied in the field of diagnosis and treatment of colorectal cancer

Inactive Publication Date: 2019-09-20
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Survivin is overexpressed in a variety of human tumors, but there is no study on the co-expression of Survivin and Aurora B in colorectal cancer

Method used

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  • Application of Aurora-B kinase and Survivin in preparation of colorectal cancer diagnosis kit
  • Application of Aurora-B kinase and Survivin in preparation of colorectal cancer diagnosis kit
  • Application of Aurora-B kinase and Survivin in preparation of colorectal cancer diagnosis kit

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Embodiment 1

[0018] Materials and methods

[0019] Patients: A total of 65 patients who underwent surgical resection of advanced colorectal cancer at Hiroshima Memorial Hospital, Japan were included in this study. In addition, 20 cases of normal colonic mucosa were used as controls. The tumors of each patient were formalin-fixed and cut into parallel 4-5mm sections, combined with hematoxylin and eosin staining, and the deepest tumor invasion site was selected by microscopic examination. This study was approved by the Ethics Committee of Hiroshima Memorial Hospital. All subjects obtained informed consent.

[0020] Immunohistochemistry. Immunohistochemical staining of Aurora B and Survivin was performed using the LSAB system (DACO, Kyoto, Japan). Briefly, paraffin blocks were cut into 3-4 μm sections. Sections were deparaffinized in xylene for 2 h, washed with graded ethanol (100-70%), dehydrated, and treated with 3% hydrogen peroxide for 30 min. Antigen retrieval was performed by auto...

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Abstract

The present invention relates to the field of diagnosis and treatment of the colorectal cancer, and in particular to the use of aurora-B kinase and survivin in the diagnosis of the colorectal cancer. The invention aims to provide application of Aurora B and Survivin in preparation of a colorectal cancer diagnosis kit. Expression of nuclear Aurora B and cytoplasmic Survivin are involved in lymph node metastasis of the colon cancer, and both nuclear Aurora B and cytoplasmic Survivin are closely related to lymph node metastasis of the colon cancer. Nuclear Aurora B and cytoplasmic Survivin are diagnostic markers and therapeutic targets for colorectal cancer.

Description

technical field [0001] The invention relates to the field of diagnosis and treatment of colorectal cancer, in particular to the use of aurora-B kinase and survivin in the diagnosis of colorectal cancer. Background technique [0002] Aurora-B kinase (Aurora-B kinase, also known as Aurora B) is a member of the serine or threonine protein kinase family that phosphorylates a variety of substrate proteins to regulate many aspects of cell division, from chromosome condensation to Cell division. It is also a protein of the chromosomal passenger complex (CPC), localized at the centromere during the transition from prometaphase to metaphase, and regulates cell division together with Survivin (Survivin), inner centromere protein (INCENP) and Borealin. Aurora B is overexpressed in various cancers such as thyroid cancer, prostate cancer, and endometrial cancer, and its upregulation is related to cell proliferation, prognosis, metastasis, and chromosome number instability, resulting in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/573
CPCG01N33/573G01N33/57419G01N33/57484
Inventor 秘思思齐广莹邵文华谈建成靳园
Owner GUILIN MEDICAL UNIVERSITY
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