Extraction method and application of endothelium corneum gigeriae galli small molecule peptide
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A technology of small molecular peptides and gallium chinensis, which is applied in the field of extraction of galanthus chinensis small molecule peptides, can solve the problems such as no reports on the production of gallic intramolecular peptides, and achieves low processing cost, simple and convenient operation steps and links. Easy-to-control effects
Pending Publication Date: 2019-09-24
山西原生肽科技有限公司
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[0004] With the development of science and technology and the application of protein engineering technology, the research and product development of bioactive small molecule peptides and their protein hydrolyzates using enzymatic hydrolysis technology has become a hot spot and mainstream. There are only a few technical documents in the laboratory, but the method for producing gallinaceous small molecule peptides in industry has not been reported
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Embodiment 1
[0030] This embodiment discloses a method for industrial production of gallinaceous gold small molecule peptide, which specifically includes the following steps:
[0031] (1) Pre-crushing of raw materials: the dried Chicken Neijin is pulverized into 80 mesh powder by a wood flour pulverizer;
[0032] (2) Dynamic extraction of slurry: use a dynamic micro-pressure extraction tank to extract, add water whose weight is 10 times that of the raw material, adjust the pH value to 9.0, raise the temperature and pressure to 100 ° C, 0.05 MPa, stir and extract under constant temperature and micro-high pressure conditions for 3-4 hour, the extract is discharged into the soup storage tank;
[0033] (3) Enzymatic hydrolysis: the extract of gallinaceous gold is discharged from the soup storage tank into the hydrolysis tank, heated and sterilized, then cooled to 50°C-52°C, added with NaOH solution to adjust the pH to 7.0, and then added dry gallinaceous gold raw material weight 0.7 % enzyme ...
Embodiment 2
[0043] This embodiment discloses a gallinaceae small-molecule peptide powder, which is prepared by the method disclosed in Example 1.
[0044] The gallinaceous gold small molecule peptide powder has the advantages of rapid absorption and high utilization rate, good solubility, stability, etc., and low allergenicity. Specifically, the gallinaceous gold small molecule peptide powder is yellow powder with a mesh number of 80 mesh, and the proportion of protein hydrolyzate with a relative molecular mass less than 1000u can reach 81%, and the protein content is 92% (calculated on a dry basis). ).
[0045] The gallinaceous gold small molecule peptide powder can be used to prepare medicines for improving gastrointestinal tract, blood lipid, protecting myocardium and the like. It can also be used to prepare health food or nutritional products.
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Abstract
The invention belongs to the technical field of biology, and particularly relates to an extraction method and application of an endothelium corneum gigeriae galli small molecule peptide. The extraction method of the endothelium corneum gigeriae galli small molecule peptide comprises the following steps that after a dry endothelium corneum gigeriae galli raw material is pre-pulverized, pulp is dynamically extracted, and then enzymatic hydrolysis and purification are carried out in sequence to obtain an endothelium corneum gigeriae galli small molecule peptide solution. The invention researches and develops the method for extracting the endothelium corneum gigeriae galli small molecule peptide through the steps of enzymatic hydrolysis and like for the first time. The method makes up for the research and development gap of the endothelium corneum gigeriae galli small molecule peptide, and adapts to the market demand and development. Through biological enzymatic hydrolysis and other technologies, endothelium corneum gigeriae galli protein is converted into a water-soluble active ingredient small molecule peptide through biological enzymatic hydrolysis, the product content is high, the absorption is complete and rapid, the operation process steps are simple and convenient, each link is easy to control, the processing cost is low, and the method can be used for industrial production.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting gallinaceous small molecular peptides and an application thereof. Background technique [0002] Gallinarum is derived from the horny inner wall of the gizzard of the chordophylum gallinae chicken, specifically refers to the inner wall of the gizzard of the domestic chicken, that is, the inner gastric membrane of the domestic chicken. Produced in all parts of the country, the source of medicine is very rich. Chicken gold is sweet and astringent in nature, flat in nature, non-toxic, and returns to the spleen, stomach, small intestine, and bladder meridian. Accumulation, enuresis, spermatorrhea, stone stranguria astringent pain, gallbladder distension hypochondriac pain and other diseases. Modern studies have shown that it mainly contains proteins, polysaccharides, amino acids, etc., and has potential biological activities in stimulating gastrointes...
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