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A kind of culture and preparation method of militaris militaris in eggs

A technology of Cordyceps militaris and eggs, which is applied in the field of cultivation and preparation of Cordyceps militaris in eggs, can solve the problems of complex cultivation process steps, long cultivation period, and limited nutritional components, and achieve the effects of short period, simple method and convenient operation

Active Publication Date: 2021-05-28
ZHOUKOU VOCATIONAL & TECHN COLLEGE +2
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Problems solved by technology

[0003] Traditional culture substrates for Cordyceps militaris are mainly rice, wheat, sorghum, silkworm chrysalis powder, etc. These types of culture substrates have limited nutrients and need to add a variety of auxiliary materials, and the traditional cultivation process is complicated and the cultivation period is long

Method used

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  • A kind of culture and preparation method of militaris militaris in eggs
  • A kind of culture and preparation method of militaris militaris in eggs
  • A kind of culture and preparation method of militaris militaris in eggs

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Embodiment 1

[0028] A method for cultivating Cordyceps militaris in eggs, characterized in that it comprises the following steps:

[0029] (1) Compatibility and optimization of solid and liquid medium: solid medium consists of 200 grams of potatoes, 20 grams of glucose, 2.5 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, 20 grams of agar, and 1000 ml of pure water. The pH is 7; the liquid medium is composed of 20 grams of glucose, 20 grams of agar, 10 grams of peptone, 3 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, and 1000 milliliters of ultrapure water. The pH of the medium is 7;

[0030] (2) Screening, optimization, rejuvenation and preservation of strains: Streak the strains of Cordyceps militaris on the slant of solid medium, and culture them in the incubator at 28°C for 7 days to obtain activated strains of Cordyceps militaris, and re- Select, pick out the colonies and inoculate them on the solid medium, and continue to cultivate t...

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Abstract

The invention provides a cultivation and preparation method of Cordyceps militaris in eggs, and relates to the technical field of fungal cultivation. The present invention selects and breeds the strains of Cordyceps militaris by optimizing the solid and liquid culture medium, and inoculates the strains of Cordyceps militaris in the allantoic cavity and yolk sac cavity of pollution-free eggs respectively according to a certain dose, under suitable temperature, humidity and dark environment After cultivating for a certain period of time, according to the inoculation dose, location and time, study the optimal production conditions of Cordyceps militaris in egg mycelium growth, establish a suitable preparation method for culture conditions, spray-dry after disinfection, crushing, beating, and filtration Process preparation Collect egg powder containing Cordyceps militaris. The process method is simple, convenient, controllable and stable, and provides technical basis and reference for industrial production of Cordyceps militaris.

Description

technical field [0001] The invention relates to the technical field of fungal culture, in particular to a method for cultivating and preparing Cordyceps militaris in eggs. Background technique [0002] With the occurrence of mixed infection of livestock and poultry infectious diseases, subclinical diseases, chronic diseases, immunosuppressive and tumor diseases, immune enhancers have become one of the most active researches in the field of applied medicine. Cordyceps militaris, also known as Cordyceps militaris and Cordyceps militaris, is similar to the active ingredients, nutritional value and pharmacological effects of Cordyceps sinensis. It has the activity of promoting macrophages and natural killer cells, regulating humoral immunity and cellular immunity, anti-tumor, protecting liver and kidney , sedation and other effects, the study of it as an immune enhancer has attracted people's attention day by day. At present, there are many medicines, health food and beauty pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 赵庆枫朱平军袁文菊杜海霞董凤华陈勇臻丁玉陈鹏举王栋
Owner ZHOUKOU VOCATIONAL & TECHN COLLEGE
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