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Sample pad treatment fluid improving analysis sensitivity of aflatoxin B1 colloidal gold detecting test strip

A technology for sample pad treatment liquid and aflatoxin, which is applied in the field of medicine and achieves the effects of improved detection sensitivity, easy availability of materials and simple operation

Inactive Publication Date: 2019-10-08
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In most cases, these two solutions can effectively improve the sensitivity of the test strip, but in some cases, it is difficult to achieve the goal of improving the sensitivity through these two technical solutions. In order to meet the performance index requirements of the kit, it is necessary to Only by adopting other technical means to improve the sensitivity can the development goal be achieved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: make the sample pad that contains 0.15% reinforcing agent

[0018] (1) Take a 1000mL reagent bottle, add 800g of pure water, weigh 12.1g of Tris, 20g of trehalose, 5g of Tween-80, 3g of TritonX-405, 2g of polyethylene glycol 300, 20g of BSA (bovine serum albumin), stack Sodium nitrogen 0.1g, sodium alginate 1.0g, dextran sulfate 0.45g, polyacrylamide 0.05g. Add the above materials into the reagent bottle one by one, each time you add a material, mix well before adding the next material. After fully mixing, adjust the pH value to 8.0 with 4M hydrochloric acid, add pure water to 1000g, and set aside;

[0019] (2) Put 5 sample pads of Austron 8964 model into the tray (length 35cm, width 28cm), take 250mL of sample pad treatment solution, pour it into the tray, make the sample pad fully soaked, and soak for 2 hours;

[0020] (3) Take out the soaked sample pad, place it horizontally in an oven, and dry it at 37°C for more than 6 hours. Cut the dry sample pad...

Embodiment 2

[0021] Embodiment 2: make the sample pad that does not contain reinforcing agent

[0022] (1) Take a 1000mL reagent bottle, add 800g of pure water, weigh 12.1g of Tris, 20g of trehalose, 5g of Tween-80, 3g of TritonX-405, 2g of polyethylene glycol 300, 20g of BSA (bovine serum albumin), stack Sodium nitrogen 0.1g. Add the above materials into the reagent bottle one by one, each time you add a material, mix well before adding the next material. After fully mixing, adjust the pH value to 8.0 with 4M hydrochloric acid, add pure water to 1000g, and set aside;

[0023] (2) Put 5 sample pads of Austron 8964 model into the tray (length 35cm, width 28cm), take 250mL of sample pad treatment solution, pour it into the tray, make the sample pad fully soaked, and soak for 2 hours;

[0024] (3) Take out the soaked sample pad, place it horizontally in an oven, and dry it at 37°C for more than 6 hours. Cut the dry sample pad to a suitable size as needed, and apply it to the test strip.

...

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PUM

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Abstract

The invention relates to a sample pad treatment fluid improving analysis sensitivity of an aflatoxin B1 colloidal gold detecting test strip. Every 1000mL of the sample pad treatment fluid is preparedby fully mixing 11-13g of a buffer solution, 20-70g of Trehalose, 5-10g of Tween-80, 5-10g of Triton X-405, 1-5g of polyethylene glycol 300, 10-40g of bovine serum albumin, 0.05-0.15g of sodium azide,0.1-1g of sodium alginate, 0.01-0.5g of dextran sulfate, 0.01-0.5g of polyacrylamide and the residues of water or deionized water, and using 4M of hydrochloric acid to adjust the pH value to 8.0. Operation is simple, the cost is low, materials are easy to obtain, the detecting sensitivity is doubled or more, and the social and economical values are high.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a sample pad treatment solution for increasing the analysis sensitivity of aflatoxin B1 colloidal gold detection test strips. Background technique [0002] Colloidal gold is a commonly used immunoassay labeling technology. It is a conventional immunolabeling technology that uses colloidal gold as a tracer marker to apply to antigens / antibodies. It is widely used in food safety testing, medical diagnosis, environmental monitoring and other fields. At the same time, it may be used in flow cytometry, electron microscopy, immunology, molecular biology and even biochips. The rapid detection test strip prepared by using this marker is a rapid detection technology based on lateral flow detection technology. Detection technology; combined with professional equipment such as colloidal gold readers, it can qualitatively and quantitatively measure various types of antigens and antibodies, ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/53G01N33/569
CPCG01N33/558G01N33/5306G01N33/56961G01N2333/38
Inventor 孙新城白艳红张华黄继红王瑞国孔红建张靖楠张艳艳冯昕魏涛刘苏萌侯佩
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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