A Vibrio alginolyticus capable of degrading various sulfonamide antibiotics and its application

A technology of Vibrio alginate and antibiotics, which is applied in the field of microorganisms and achieves the effects of low cost, wide range of use, and easy large-scale promotion

Active Publication Date: 2019-12-24
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports on the degradation and metabolism of sulfa antibiotics by Vibrio

Method used

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  • A Vibrio alginolyticus capable of degrading various sulfonamide antibiotics and its application
  • A Vibrio alginolyticus capable of degrading various sulfonamide antibiotics and its application
  • A Vibrio alginolyticus capable of degrading various sulfonamide antibiotics and its application

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Embodiment Construction

[0018] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0019] 1. Strain screening

[0020] 1. Enrich the strains that may have the degradation effect of sulfonamide antibiotics

[0021] Prepare MM medium: 1 g ammonium nitrate, 0.5 g potassium dihydrogen phosphate, 1.5 g disodium hydrogen phosphate, 1 g sodium chloride, 0.2 g magnesium sulfate heptahydrate, 1 L distilled water, adjust pH to 7.0-7.5, 120 °C Autoclave for 15 min.

[0022] On May 26, 2018, we took 2L of estuary water (including 2g of sediment) from the estuary of Yantai's wandering river, and filtered and enriched the estuary water on a 0.44μm filter membrane under sterile conditions. Membrane and sediment samples were added to 200mL MM medium containing 4mg sulfamethoxazole, cultured in a constant temperature incubator at 30°C with 140rpm shaking for 5 days, then sucked 2mL of turbid culture solution, and added it to 200mL new...

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Abstract

The invention discloses a Vibrio alginolyticus capable of degrading various sulfonamide antibiotics and its application, wherein the Vibrio alginolyticus is screened from the estuary water of the Yantai Wandering River estuary, and was released in June 2019 On the 26th, it was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. The preservation number is: CGMCC No.18031. It has the ability to degrade sulfonamide antibiotics and can degrade sulfadiazine, sulfisoxazole, sulfamethoxazole, A variety of sulfonamide antibiotics including methylazine, sulfamethoxine, sulfacetamide, sulfamethazine, sulfathiazole, and sulfapyridine. The benefit of the present invention is that: the Vibrio alginolyticus (preservation number is CGMCC No.18031) screened by the present invention has great advantages in the treatment of polluted water (including fresh water and sea water) containing high concentrations of sulfonamide antibiotics. Application prospect.

Description

technical field [0001] The invention relates to a strain of vibrio and its application, in particular to a strain of vibrio alginolyticus capable of degrading various sulfonamide antibiotics and its application, and belongs to the technical field of microorganisms. Background technique [0002] Sulfonamides is a general term for a class of drugs with p-aminobenzenesulfonamide structure. It is a class of artificially synthesized broad-spectrum antibacterial drugs, which can compete with p-aminobenzoic acid in bacteria for dihydrofolate synthase, inhibit Synthesis of dihydrofolate, thereby inhibiting the growth and reproduction of bacteria. [0003] Sulfonamide antibiotics are widely used in animal husbandry, aquaculture and medical industries because of their advantages such as wide antibacterial spectrum, stable properties, many varieties, and low price. Metabolism, more than 80% of sulfonamide antibiotics will enter the environment through excretion. In recent years, the d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/63C02F101/38C02F103/08
CPCC02F3/34C02F2101/40C02F2103/08C12N1/205C12R2001/63
Inventor 陈令新王巧宁姜亚茹吕敏王虹丹
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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