Endophytic fungus capable of promoting schima superba seedling height and ground diameter growth under low phosphorus environment

A technology of endophytic fungi and wood lotus, applied in the field of microorganisms to achieve the effect of promoting growth and plant growth

Active Publication Date: 2019-10-18
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Scholars at home and abroad have screened a variety of endophytic fungi with growth-promoting and phosphorus-dissolving effects from plants. There are few reports on the screening and identification of fungi

Method used

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  • Endophytic fungus capable of promoting schima superba seedling height and ground diameter growth under low phosphorus environment
  • Endophytic fungus capable of promoting schima superba seedling height and ground diameter growth under low phosphorus environment
  • Endophytic fungus capable of promoting schima superba seedling height and ground diameter growth under low phosphorus environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Isolation of endophytic fungi of Schima superba

[0014] 1. Main equipment

[0015] Ultra-clean workbench SW-CJ-1FD, constant temperature incubator HH•B11-II, constant temperature culture oscillator zhwy-211B, ten-thousandth balance AR1140, automatic vertical sterilizer LMQ.C-4060, ultrapure water Machine P60-CW etc.

[0016] 2. Main Reagents and Media

[0017] Reagents: 15% sodium hypochlorite, 75% absolute ethanol, primer PAGE 11-59bp OD 1-2, DNA electrophoresis loading buffer, GoodViewTM nucleic acid dye, 2×Tap PCR MasterMix, fungal DNA extraction kit, DNA purification and recovery kit.

[0018] Medium: (1) Modified Martin agar medium: peptone 5.0g, yeast extract powder 2.0g, glucose (C 6 h 12 o 6 •H 2 O) 20.0g, Potassium dihydrogen phosphate (KH 2 PO 4 ) 1.0 g, magnesium sulfate (MgSO 4 •7H 2 O) 0.5g, agar 15.0g, ultrapure water 1000ml, pH 6.2~6.6.

[0019] (2) Improved Martin's liquid medium: peptone 5.0g, yeast extract powder 2.0g, glucose (C ...

Embodiment 2

[0045] Preparation of bacterial solution: Inoculate the activated phosphate-solubilizing bacteria into 40 mL of modified Martin liquid medium, and culture on a constant temperature shaker for 72 h (28 °C, 160 r min -1 ). Calculate and calculate the number of spores with the blood count method, and dilute the cultivated bacterial solution with sterile water to 5.5×10 6 pcs L -1 .

[0046] This experiment adopts soil cultivation pot experiment, and the seedlings of Schima superba selected for the experiment are annual seedlings, with an average seedling height of 20cm and an average ground diameter of 3.0mm, provided by Fujian Academy of Forestry Sciences. On March 3, 2016, Schima superba seedlings with consistent growth were selected and planted in plastic pots with a diameter of 15 cm and a height of 10 cm. The soil required for the experiment was mixed and weighed, and an equal amount (4kg) of yellow core soil was placed in each pot. The nutrient content in the soil is sho...

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Abstract

The invention discloses an endophytic fungus capable of promoting schima superba seedling height and ground diameter growth under low phosphorus environment. The endophytic fungus MG37 is Botryosphaeria sp., which is registered and preserved on April 8, 2019 at the General Microbiology Center of the China Microbial Culture Collection Management Committee, and a preservation number is CGMCC No. 17477. The strain is isolated and purified from the root of Schima superba, which can alleviate the restriction of phosphorus stress condition on plant seedling height and ground diameter growth, and canpromote the schima superba seedling height and ground diameter growth under the low phosphorus environment so as to promote the plant growth.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an endophytic fungus capable of promoting the growth of the height and ground diameter of Schima superba seedlings in a low-phosphorus environment. Background technique [0002] Phosphorus is one of the three major nutritional elements needed for plant growth and development. Phosphorus deficiency will affect the metabolic processes of plant photosynthesis, material energy conversion and other life activities. The soil in southern my country is generally deficient in phosphorus, and the phosphorus in the soil is almost in the form of invalid phosphorus, and the part that can be absorbed and utilized by plants is very small. In a low-phosphorus environment, plants will evolve a series of response mechanisms to alleviate the constraints of insufficient phosphorus supply on their growth, such as increasing the absorption and utilization efficiency of available phosphorus in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
CPCC12R2001/645C12N1/145A01N63/30
Inventor 李键徐欢吴承祯林勇明林晗洪滔
Owner FUJIAN AGRI & FORESTRY UNIV
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