Method and system for reconstructing microscopic fluorescence image of thick sample
A micro-fluorescence and image reconstruction technology, which is applied in image enhancement, image analysis, image data processing, etc., can solve the problems of insufficient overall clarity, difficulty in collecting fluorescence images, and inability to achieve clear collection of fluorescence features, so as to improve fluorescence Image quality and overall clarity, resolving indeterminate effects
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[0056] The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present invention. These all belong to the protection scope of the present invention.
[0057] According to a method for reconstructing a microscopic fluorescence image of a thick sample provided by the present invention, the method comprises the following steps:
[0058] Data cube acquisition step: In the same field of view, within the range of the fluctuation of the fluorescent slice, the field of view images of different focal planes are collected at equal intervals with a step length smaller than the depth of field of the microscope to obtain a data cube;
[0059] Partial grid division...
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