A kind of resistance identification method of sweet potato leaf roll virus disease

Abstract

The invention discloses a resistance identification method for sweet potato leaf-rolling virus disease, comprising the following steps: after the identified varieties are cultured and molecularly detected by detoxification test-tube seedlings, the original seedlings that do not contain leaf-rolling virus disease SPLCV; high-multiplication of leaf-roll virus indicator plants; identification of virus-free tissue culture seedlings isolation, insect vector inoculation under airtight conditions; identification of plant grafting and inoculation under the condition of net room; yield comparison test planting, harvesting, yield measurement, and seed conservation ; Seed in the second year, investigate and identify the emergence rate of leaf-rolling virus disease of the varieties; evaluate the resistance (resistance) of the varieties to leaf-rolling virus. The invention is an identification method for anti-sweet potato leaf roll virus disease (SPLCV), which utilizes the transmission law of the virus to carry out natural virus transmission, insect vector inoculation and grafting inoculation for the identified varieties in different periods, and through the seedbed performance of each variety in the second year , according to the grading standard, to determine the resistance, tolerance and susceptibility characteristics of varieties to sweet potato leaf roll virus disease, and to provide basic resistance sources for the selection and breeding of sweet potato leaf roll virus disease-resistant varieties.

Description

technical field [0001] The present invention relates to a resistance identification method for sweet potato leaf roll virus disease. Background technique [0002] As the seventh largest food crop in the world, sweet potato is mainly distributed in Asia, Africa, Latin America and other regions. China's planting area accounts for about 80% of the world's total. However, due to the widespread existence of sweet potato virus disease, the yield and quality of sweet potato are seriously affected. According to some data, the incidence of sweet potato virus disease in field varieties is 100%. In East Africa and South America, the production of sweet potato has been greatly reduced or even stopped due to virus disease. Disease is an important disease in sweet potato producing areas in China, and it is an important cause of sweet potato degradation and yield reduction. The infection of virus disease can reduce the yield by 30% to 50%. The sweet potato after removing the virus can gr...

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Problems solved by technology

The above three methods have certain feasibility for the evaluation of virus resistance of some viruses in sweet potato varieties, but there are many problems in the identification of sweet potato leafroll virus, which mainly uses insects as mediators, because there are two types of virus resistance performance of varieties: one is variety It has anti-virus genes, and the other is to reduce insect-borne viruses and avoid diseases

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