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Coptidis rhizome reference extract and preparation method and application thereof

A Coptis chinensis extract and extract technology are applied in the field of chemistry and can solve the problems of high detection cost and incomplete detection standards.

Inactive Publication Date: 2019-10-25
广州万正药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the above-mentioned technical problems, the object of the present invention is to provide a kind of in order to solve the above-mentioned technical problems, the detection standard that the present invention exists in the Coptis Rhizoma preparation or the Coptis Rhizoma medicinal material detection is not comprehensive, the problem that uses a variety of reference substances to detect the problem that cost is high, the present invention Provided is a Coptidis rhizome control extract that simultaneously detects compounds in Coptis chinensis, Coptis chinensis extract and Coptis chinensis preparations. The Coptis chinensis control extract has good consistency, stable and uniform properties, and can be used for quality control of Coptis chinensis and Coptis chinensis prescriptions , qualitative and quantitative analysis and determination of effective compounds

Method used

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  • Coptidis rhizome reference extract and preparation method and application thereof
  • Coptidis rhizome reference extract and preparation method and application thereof
  • Coptidis rhizome reference extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Embodiment 1, the comparison test of the Coptis Rhizoma control extract of the present invention instead of the control medicinal material

[0080] Commercial medicinal materials were analyzed by thin-layer chromatography and high-performance liquid chromatography with chemical reference substance and Coptis rhizome reference extract as reference substances.

[0081] 1 TLC

[0082] 1.1 Sample preparation

[0083] Chemical reference solution: accurately weigh berberine, and prepare a 0.1 mg / ml solution with methanol; accurately weigh jatrorrhizine, epiberberine, coptisine and palmatine, respectively, and prepare 0.2 mg with methanol / ml solution.

[0084] Coptidis control extract solution: Accurately weigh 60 mg of the Coptidis control extract prepared in Example 1, add 20 ml of methanol, ultrasonically (500w) for 15 minutes, shake well, pass through a 0.22 μm filter membrane to obtain the Coptidis control extract solution).

[0085] Medicinal materials for test solut...

Embodiment 2

[0117] Embodiment 2, the character analysis of Coptis Rhizoma control extract

[0118] 1. Apparent state: the Coptidis rhizome control extract obtained in Example 3 is a yellow powder.

[0119] 2. Moisture determination: Carry out according to Appendix IX G of the Chinese Pharmacopoeia of the 2010 edition (reduced pressure drying method). The test result shows that the water content of the Coptis rhizome control extract is 6.45%.

[0120] 3. Consistency test: 10 batches of Coptidis rhizome control extracts were prepared according to the method in Example 1, and the TLC detection results for epiberberine, coptisine, palmatine and berberine were determined between each batch The difference is very small, and the fluorescent spots of epiberberine, coptisine, palmatine and berberine are all clearly displayed, and the distribution is very consistent; the epiberberine, coptisine, palmatine The contents of berberine and berberine are within the standard range of deployment. Theref...

Embodiment 3

[0125] Embodiment 3, the preparation of Coptis Rhizoma control extract

[0126] Instruments and reagents: Linomat 5 thin-layer chromatography semi-automatic spotting instrument, ATS 4 thin-layer chromatography automatic spotting instrument, thin-layer chromatography double-slot expansion cylinder, Chromatogram Immersion Device III chromatographic immersion tank and TLCvisualizer thin-layer chromatography camera instrument (Switzerland, CAMAG).

[0127] Agilent 1260series high performance liquid chromatograph equipped with DAD detector (AgilentTechnologies, USA).

[0128] Methanol, ethanol, and isopropanol were all analytically pure (Tianjin Damao Chemical Reagent Factory). Toluene, ethyl acetate, butyl acetate, concentrated ammonia and triethylamine were of analytical grade (Guangzhou Chemical Reagent Factory).

[0129] Berberine hydrochloride (also known as berberine), palmatine hydrochloride (also known as palmatine in the text), coptisine, epiberberine, all marked purity ...

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Abstract

The invention discloses a coptidis rhizome reference extract and a preparation method and application thereof. The extract is yellow dry powder, which is prepared from 67 to 84 percent by weight of acoptidis rhizome extract and 16 to 33 percent of an auxiliary ingredient. The coptidis rhizome extract contains not less than 18 percent of jateorhizine, palmatine, berberine, epiberberine and coptisine. The coptidis rhizome reference extract is high in consistency and stable and uniform in character, can be used for quality control of coptidis rhizome medicinal materials and coptidis rhizome preparations, and can qualitatively and quantitatively analyze and measure effective compounds.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a Coptis rhizome control extract and a preparation method thereof. Background technique [0002] Drug inspection is an important means to verify the quality of drugs. With the widespread use of instrumental analysis, more and more drug reference materials are used. In drug inspection work, reference substances are often used as a special reference for checking the quality of drugs, and as a comparison standard for determining the authenticity of drugs. Reference substances play a very important role in drug testing. [0003] In the current standard of traditional Chinese medicine, the quality control mode of a single component is difficult to reflect the quality of traditional Chinese medicine, and the method of comprehensive quality control using multiple components or characteristic chromatographic peaks has attracted more and more attention. However, the shortage of comm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/04G01N30/90
CPCG01N30/02G01N30/04G01N30/90G01N2030/042
Inventor 许舜军郭隆钢许艺镌
Owner 广州万正药业有限公司
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