New application of black bean peel anthocyanin extract to antagonism of organism damage caused by lead exposure

A skin anthocyanin and extract technology is applied in the field of antagonism and intervention effect of nutrient intake on environmental poison exposure damage, and achieves the effect of improving iron metabolism and exercise ability.

Pending Publication Date: 2019-11-12
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, chronic lead poisoning may be associated with anemia, some of which is characterized by iron deficiency, thought to be the result of inhibition of heme synthase, which inhibits iron uptake by red blood cells

Method used

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  • New application of black bean peel anthocyanin extract to antagonism of organism damage caused by lead exposure
  • New application of black bean peel anthocyanin extract to antagonism of organism damage caused by lead exposure
  • New application of black bean peel anthocyanin extract to antagonism of organism damage caused by lead exposure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Obtaining and structure identification of anthocyanin extract from black bean hull (especially the purity value)

[0025] Weigh an appropriate amount of black soybean skin, the ratio of solid to liquid is 1:30, and the extractant is 60% ethanol with a pH of 1. The extraction temperature was 60°C, and the extraction time was 120 minutes. The extract is concentrated by centrifugation, purified by AB-8 macroporous adsorption resin, and vacuum freeze-dried to obtain the black soybean hull extract. The anthocyanin content in the extract is about 200mg / g; the identification by high performance liquid chromatography shows that the main anthocyanin component in the anthocyanin sample of black bean hull is cyanidin 3-O-glucoside.

Embodiment 2

[0027] Grouping and treatment conditions of Drosophila

[0028] Drosophila were normally cultured in an incubator with a culture condition of 25°C and 60% humidity. The lead treatment condition is: add 100mM lead acetate to the feed; the addition condition of black soybean hull anthocyanin extract is: 2mg / mL basal medium; the addition condition of black soybean hull powder is: 20mg / mL.

Embodiment 3

[0030] Survival rate, climbing ability and lifespan detection of Drosophila

[0031] The method for detecting the survival rate of fruit flies is as follows: in each food treatment group, 50 first-instar larvae are selected in each group, and the number of fruit flies that can successfully hatch in each group is counted. Experimental results such as figure 1 As shown, lead exposure can significantly reduce the eclosion rate of Drosophila larvae, indicating that it has caused significant damage to the survival rate of Drosophila, and this defect can be rescued by the treatment of black soybean hulls or black soybean hulls anthocyanin extracts.

[0032] The method for detecting the climbing ability of fruit flies is as follows: in each food treatment group, each group picks 20 fruit flies in a 20 cm long empty tube, knocks all the fruit flies into the bottom, and counts them for 5 seconds. Drosophila can climb a number of 10 cm high. Experimental results such as figure 2 As ...

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Abstract

The invention discloses a new application of a black bean peel anthocyanin extract to antagonism of organism damage caused by lead exposure, and belongs to the technical field of a new application ofactive substances. Acute and chronic lead exposure can cause a series of behavioral deficiency and functional damage of organisms, and black bean peel and anthocyanin components thereof have multipleefficacy of eliminating oxygen free radicals, resisting inflammation, resisting tumors, preventing aging, reducing blood sugar, reducing blood lipid, preventing cardiovascular diseases and the like. The invention discloses a technical scheme that the black bean peel anthocyanin extract disclosed by the invention can effectively relieve damage of fruit fly life, action and the like caused by lead exposure, and further limits a technical scheme that the black bean peel anthocyanin extract exerts beneficial effects through stable regulation on the iron metabolism of organisms. The invention provides a nutrient scheme of applying black bean peel anthocyanin extract to antagonism of lead damage for the first time, through detection of a plurality of indexes, notable technique effects of the black bean peel anthocyanin extract are confirmed, further development of the new application value of the black bean peel and the anthocyanin extract thereof is facilitated, and a new scheme is providedfor intervention, prevention and relief of lead exposure damage through food and nutrient absorption.

Description

technical field [0001] The invention belongs to the technical field of new uses of active substances, and in particular relates to a new use of antagonism and intervention effects of nutrient intake on environmental poison exposure damage. Background technique [0002] Exhaust gas heavy metal lead is a widely distributed environmental pollutant. As a xenobiotic, lead does not have any beneficial physiological functions. Its acute and chronic exposure can cause varying degrees of damage to multiple systems of the body. life. Since it exists widely in the environment and chronic exposure can affect the physiological function and behavior of the body for a long time, long-term intervention and prevention through selective intake of nutrients has become an option for antagonizing lead damage. Program. [0003] Black beans, also known as black beans, are native to China. They are a good product for both food and medicine. They have relatively high nutritional value and are blac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K36/48A61P39/02
CPCA61K31/352A61K36/48A61P39/02
Inventor 肖桂然徐毅李飞翔
Owner HEFEI UNIV OF TECH
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