Deer heart protein hydrolyzate and its preparation method and application

Abstract

The invention relates to a deer heart protein hydrolyzate, a preparation method and application thereof, and belongs to the technical field of biomedicine. The preparation method comprises the following steps: pretreatment: taking deer heart, adding physiological saline, and homogenizing tissue to obtain an enzymolysis stock solution for use; enzymolysis: taking the above enzymolysis stock solution, adding pepsin for enzymolysis reaction, and obtaining Enzymatic hydrolysis solution; purification: take the above enzymatic hydrolysis solution, separate and purify it with a Sephadex column, and take a 3KD-15KD polypeptide to obtain it. In the present invention, the heart of sika deer is used as raw material, and the small molecular polypeptide substance is obtained through tissue homogenization and a suitable enzymatic hydrolysis method. And the aging cardiomyocyte model was established, and the effect of various enzymatic hydrolyzates on the proliferation rate of cardiomyocytes was detected by MTT method. The experiment confirmed that the obtained deer heart protein hydrolyzate has good activity in alleviating the aging of cardiomyocytes.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a deer heart protein hydrolyzate and its preparation method and application. Background technique [0002] Sika deer (Cervus nippon) is a rare medicinal animal that is special in the monsoon region of East Asia. It is used for meat, skin and ornamental, and has high economic value. Deer heart is a good product for invigorating qi and calming the nerves. According to the traditional Chinese medicine theory of nourishing shape, deer heart can treat rheumatic heart disease, heart failure, palpitation and palpitation and other diseases related to the heart. With its unique nutritional value and traditional The habit of eating deer heart has always been favored by experts and scholars, and it has become a hot spot in the study of heart disease and anti-aging. [0003] However, the sika deer heart is used in the folk compatibility of Chinese herbal medicines, in the form of pills ...

AI Technical Summary

Problems solved by technology

The traditional deer heart is often used as a precious medicinal material by humans to cook into delicacies. At present, there is no in-depth research on sika deer in modern medicine.

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