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Application of reagent for detecting immune function index, detection kit for recurrent abortion, and method for detecting immune function index

A detection kit and recurrent miscarriage technology, applied in the field of biomedicine, can solve the problems of one-sided information, inability to evaluate immune function, and insufficient in-depth evaluation

Inactive Publication Date: 2019-11-15
江西普锐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, these tests only evaluate one or a small class of immune factors, and the evaluation is not deep enough. The information obtained is very one-sided, and it is impossible to give a comprehensive assessment of the mother's immune function.

Method used

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  • Application of reagent for detecting immune function index, detection kit for recurrent abortion, and method for detecting immune function index
  • Application of reagent for detecting immune function index, detection kit for recurrent abortion, and method for detecting immune function index
  • Application of reagent for detecting immune function index, detection kit for recurrent abortion, and method for detecting immune function index

Examples

Experimental program
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Effect test

Embodiment 1

[0039] In this example, the peripheral blood samples of 62 cases of nulliparous population, 60 cases of peripheral blood samples of fertile population, and 34 cases of peripheral blood samples of recurrent abortion population (these samples were all from hospitals and provided by clinicians) were analyzed. Function, mainly for immune cells CD4 in peripheral blood + , CD8 + The functional indicators of T cells, NK cells and γδT cells were analyzed.

[0040] Results: Through the detection and analysis of a large number of clinical samples, we found that 12 immune indicators are highly correlated with the risk of recurrent miscarriage. The 12 immune indicators are: total T cells, double positive T cells, peripheral helper Tfh cells, terminally differentiated CD4 + T cells, central memory CD4 + T cells, Tc2 subset CD8 + T cells, potentially functional CD8 + T cells, activated NK cells, immature NK cells, mature NK cells, immature / mature NK cell ratio, and NKP30-positive Vδ2 s...

Embodiment 2

[0043] The present embodiment provides a recurrent miscarriage detection kit, which includes the following monoclonal antibodies: anti-CD3 monoclonal antibody, anti-CD4 monoclonal antibody, anti-CD8 monoclonal antibody, anti-CD28 monoclonal antibody, anti-CD45RA monoclonal antibody, anti- CCR7 monoclonal antibody, anti-γδ monoclonal antibody, anti-CD56 monoclonal antibody, anti-NKG2D monoclonal antibody, anti-NKP30 monoclonal antibody and anti-Vd2 monoclonal antibody. Each of the above monoclonal antibodies carries a fluorescent dye.

[0044] Fluorescent dyes selected from: APC-Cy7, APC-R700, AF647, BV510, PE, BV421, BB515, BV605, PE-Cy7, BB700, Alexa Fluor 350-790, Cy3, Cy5, Dylight 488-680, PE-Alexa Fluor Any one of , PE-Cy5 and PE-Cy7. Each antibody can be purchased on the market, and its fluorescent dye can be selected according to the detection needs, and different antibodies have different fluorescent dyes.

[0045] The preparation method of the above-mentioned kit: pr...

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Abstract

The invention discloses an application of a reagent for detecting an immune function index, a detection kit for recurrent abortion, and a method for detecting the immune function index, and relates tothe biomedicine field. The invention discloses the application of the reagent for detecting the immune function index for preparing the detection kit for the recurrent abortion. The immune function index comprises the following indexes: a total T cell, a double-positive T cell, a peripheral auxiliary Tfh cell, a terminally differentiated CD4+T cell, a central memory CD4+T cell, a Tc2 subpopulation CD8+T cell, a potential functional CD8+T cell, an activated NK cell, an immature NK cell, a mature NK cell, an immature / mature NK cell ratio and an NKP30 positive V[delta]2 subpopulation[gamma][delta]T cell. By use of the application, the analysis of various samples are discovered for the first time, parts of immune function indexes are highly related to the recurrent abortion, and therefore, the reagent for detecting the immune function indexes can be used for preparing the detection kit for the recurrent abortion.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of reagents for detecting immune function indexes and a recurrent miscarriage detection kit and method. Background technique [0002] The immune function of human immune cells is not only directly related to various diseases (such as cancer and autoimmune diseases), but also closely related to female reproductive health. It is generally considered that pregnancy is a kind of allogeneic transplantation, and the normal growth of the embryo depends on the maintenance of its immune tolerance state in the mother. Once this state is broken, it will lead to miscarriage. The state of immune tolerance of the body depends on the functional state of immune cells in the body, so the function of immune cells is an important determinant of whether the mother can have a normal pregnancy and whether she will have repeated miscarriages. [0003] Recurrent abortion (RSA) generally refer...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/68
CPCG01N33/577G01N33/6893G01N2800/36
Inventor 吴扬哲廖爱华尹芝南高伊昉
Owner 江西普锐生物科技有限公司