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Fully-human-derived CAR-T preparation and preparation method thereof

A fully human source and preparation technology, applied in DNA preparation, biochemical equipment and methods, chemical instruments and methods, etc.

Inactive Publication Date: 2019-11-22
WUHAN YONGSEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to provide a high-intensity, high-precision, fully human-derived CAR-T that can meet the needs of round tubes for the recurrence problem after existing CAR-T treatment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Using gene display technology (mRNA Display technology), the fully human-specific scFv was screened out. All CAR-Ts are not mouse-derived, and fully human-derived CAR-T is superior to the mouse-derived CAR-T currently on the market or in clinical trials, and will reduce immunogenicity. Fundamental to this technique is the selection of the target. Targets CD19Δex2, CD22 and FcRH5 are all expressed on living cells and can maintain complete antigenicity, while the gene display technology mRNA Display technology can use antibody gene libraries to produce specific antibody scFv against targets on the surface of living cells such as CD19Δex2 The most critical part of CAR-T.

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PUM

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Abstract

The invention discloses a fully-human-derived chimeric antigen receptor-T cell (CAR-T) preparation and a preparation method thereof. The method utilizes an mRNA display technology to screen out a fully-human-derived specific scFv. All CAR-T are not mouse-derived, and the fully-human-derived CAR-T is better than a mouse-derived CAR-T currently on the market or in clinical trials and can reduce immunogenicity. The essence of the technology provided by the invention is the choice of target spots, target points CD19deltaex2, CD22, and FcRH5 are all expressed on living cells and can maintain complete antigenicity, the mRNA display technology can use an antibody gene library to generate the specific antibody scFv aiming at target points of the surfaces of the living cells such as CD19deltaex2 toprepare a most critical part of the CAR-T, and the method has a better effect on tumor treatment and great significance.

Description

technical field [0001] The invention relates to a connection structure between components, in particular to a circular pipe connection structure and a special loading and unloading tool thereof. Background technique [0002] Artificially prepare specific antibodies against tumor targets, and then use genetic engineering technology to replace the variable region of the T cell receptor (TCR) with the region of the antibody with binding function, forming TCR and antibody chimera ( C Himeric A ntigen R eceptor, CAR), using viral vectors, added to T cells outside the human body, antibodies can be expressed on the surface of T cells, this kind of T cells containing chimeric antibodies is called CAR-T cells (CAR plus T cells). The full name in Chinese is "Chimeric Antigen Receptor Modified T Cells". When the CAR-T cells are infused back into the patient, the antibody can recognize and specifically bind to the target on the tumor cells, activate the T cells and make them kill the...

Claims

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Application Information

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IPC IPC(8): C12N5/10C12N15/10A61K35/17A61P35/00
CPCA61K35/17A61P35/00C07K16/2803C07K16/283C07K2317/24C07K2317/622C12N5/0636C12N15/1041C12N2510/00
Inventor 张克明欧阳晨曦
Owner WUHAN YONGSEN BIOTECH