Method for quickly and qualitatively detecting antibacterial performance of filter material
A filter material and qualitative detection technology, which is applied in the direction of microbial-based methods, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of inconspicuous results, material antibacterial ability description, etc., and achieve wide applicability and detection The effect of low cost and convenient operation
Pending Publication Date: 2019-11-26
SHENZHEN ANGEL DRINKING WATER IND GRP
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Problems solved by technology
This method is relatively convenient to operate, but the result is not obvious when it is used to detect the filter material loaded with non-elutable antibacterial components, and it is difficult to explain the antibacterial ability of the material
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[0022] In order to make those skilled in the art more clearly understand the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and embodiments.
[0023] In one embodiment of the present invention, the method for rapid qualitative detection filter material antibacterial property comprises the following steps:
[0024] S1, take the test bacteria to prepare the test bacteria solution.
[0025] Specifically, take the agar medium of Escherichia coli, wash the 24h slant culture with PBS buffer, and make 10-fold incremental dilutions successively, and the concentration of the bacterial solution is selected as 3.0×10 3 CFU / mL~5.0×10 3 The dilution of CFU / mL was used as the bacterial solution for the test. It should be noted that, in other embodiments of the present invention, the test bacteria can be Escherichia coli, Staphylococcus aureus or other designated bacte...
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The invention discloses a method for quickly and qualitatively detecting the antibacterial performance of a filter material. The method for quickly and qualitatively detecting the antibacterial performance of the filter material comprises the following steps that a test bacterial solution is prepared by taking test bacteria; a filter material to be tested and a control filter material are prepared, and sterilization treatment is carried out; the sterilized filter material to be tested and the sterilized control filter material are taken to filter the test bacterial solution, the filter material to be tested and the control filter material which are subjected to the test bacterial solution filtering are pasted on an agar medium, the medium is inverted, and the mixture is cultivated under the condition of 37+ / -1 DEG C for 18-48 h; and the sterilization effect is observed, the growth of bacterial colonies on the periphery of the filter material to be tested and the control filter materialis checked, and the antibacterial effect is evaluated. According to the method for quickly and qualitatively detecting the antibacterial performance of the filter material, the filter material to betested and the control filter material are used for separately filtering the test bacterial solution, then the filtered filter material is used for patch cultivation, and finally the antibacterial performance of the filter material is quickly determined through observation and comparison. The method is reasonable in design, convenient to operate, low in detection cost and wide in applicability.
Description
technical field [0001] The invention relates to the technical field of material detection, in particular to a method for rapidly qualitatively detecting the antibacterial performance of filter materials. Background technique [0002] Due to safety and health concerns, the research on the antibacterial properties of filter materials has received more and more attention. At present, the vibration method and the inhibition zone method are often used to detect the antibacterial properties of materials. The shaking method is to add the bacterial solution and the material to be tested together into the PBS buffer for a certain period of time, and then compare the concentration of the bacterial solution after shaking with the initial concentration of the bacterial solution, so as to quantitatively evaluate the antibacterial performance of the material to be tested. In the patent CN201610011352.6 and the patent CN201110249902.5, the shock method is used in the pre-operation. This me...
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Login to View More IPC IPC(8): C12Q1/18C12Q1/10C12Q1/14C12R1/19C12R1/445
CPCC12Q1/18C12Q1/10C12Q1/14
Inventor 马依文李国平骆霁月孟繁轲夏雪
Owner SHENZHEN ANGEL DRINKING WATER IND GRP