A ratiometric fluorescent molecularly imprinted paper chip and its preparation method and application

A fluorescent molecular imprinting, paper chip technology, applied in fluorescence/phosphorescence, material analysis by optical means, material analysis, etc., can solve the problems of measurement value and true value error, poor selectivity, etc., to achieve rapid detection and improve selectivity Effect

Active Publication Date: 2022-02-08
陕西国环绿城环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although carbon quantum dots have good fluorescence characteristics, as far as carbon quantum dots are concerned, as a fluorescent probe, its biggest disadvantage is poor selectivity.
In addition, fluorescent probes in the prior art are generally only detected according to a change in the fluorescent signal, which easily leads to the problem of errors between the measured value and the real value

Method used

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  • A ratiometric fluorescent molecularly imprinted paper chip and its preparation method and application
  • A ratiometric fluorescent molecularly imprinted paper chip and its preparation method and application
  • A ratiometric fluorescent molecularly imprinted paper chip and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method of preparation of a ratio type fluorescent molecule imprinted paper chip, such as figure 1 As shown, including the following steps:

[0036] A.CDS Synthesis: It is sufficient to add 0.15 g of folic acid powder to 50 ml of secondary water, thoroughly stirred, and then transferred to a solution at 180 ° C at 180 ° C for 2 h in the reaction kettle, and naturally cooled after completion. The synthetic CdS was purified by the G-25 glucan gel column by G-25 glucan gel column, and the obtained pure product CDS was treated with tin foil paper and placed in the darkness for use;

[0037] B. CDS Modification: Take 7.128 mgl-Cathogen Add 18 ml of the synthesized CDS solution described in step A, under the heat-conducting conditions, at 37 ° C constant temperature for 40min after 40 minutes;

[0038]c. Synthesis of cellulose fluorescent paper with CDS: First, in a petri dish, a cut and sized cellulose paper is placed, 40 ml of 0.2% dilute hydrochloric acid is acidified, oscill...

Embodiment 2

[0044] A method of preparation of a ratio-type fluorescent molecular imprint paper chip, including the following steps:

[0045] A.CDS Synthesis: It is weighed into 40 ml of secondary water at 0.15 g of folic acid powder, stirred well, then transferred to a solution at 170 ° C for 2 h at 170 ° C in the reactor, and naturally cooled after completion. The synthetic CdS was purified by the G-25 glucan gel column by G-25 glucan gel column, and the obtained pure product CDS was treated with tin foil paper and placed in the darkness for use;

[0046] B. CDS Modification: Take 7.128 mgl-Cathylcysteine ​​Add 18 ml of the synthesized CDS solution described in step A, under the heat-conducting conditions, at 36 ° C for 35min after 35 minutes;

[0047] c. Synthesis of cellulose fluorescent paper with CDS: First, in a petri dish, a cut and sized cellulose paper is placed, and 35 ml of 0.2% dilute hydrochloric acid is acidified. After oscillating 15 min, it is flushed with deionized water thre...

Embodiment 3

[0053] A method of preparation of a ratio-type fluorescent molecular imprint paper chip, including the following steps:

[0054] Synthesis of A.CDS: It is sufficient to add 0.15 g of folic acid powder to 60 ml of secondary water, thoroughly stirred, and then transferred to a solution at 190 ° C at 190 ° C for 3 h in the reaction kettle, and naturally cooled after completion. The synthetic CdS was purified by the G-25 glucan gel column by G-25 glucan gel column, and the obtained pure product CDS was treated with tin foil paper and placed in the darkness for use;

[0055] B.CDS modification: Take 7.128 mgl-cysteine ​​Add 20 ml of the synthesized CDS solution described in step A, under the heat-precipitation conditions, at 38 ° C for 45min after 45 minutes;

[0056] c. Synthesis of cellulose fluorescent paper with CDS: First, in a petri dish, a cut and sized cellulose paper is placed, 45 ml of 0.2% dilute hydrochloric acid is acidified, and oscillate 25min, rinse three times with dei...

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Abstract

The invention provides a ratio-type fluorescent molecularly imprinted paper chip and its preparation method and application, belonging to the field of material science and engineering and microfluidic chip technology, comprising the following steps: sequentially modifying CDs and APTES-NBD conjugate fluorescent materials On the surface of cellulose paper, difenoconazole imprinted holes were formed on the surface of cellulose paper by surface imprinting technology to prepare fluorescent molecularly imprinted paper, which was immobilized on the microfluidic substrate with three-dimensional structure to obtain Ratio-type fluorescent molecularly imprinted paper chip; the invention realizes the rapid detection of the pesticide difenoconazole, and develops a microfluidic rapid detection paper chip with the characteristics of low toxicity, portability, high efficiency, and economy.

Description

Technical field [0001] The present invention belongs to the field of material science engineering and microfluidic chip technology, and specific relates to a ratio fluorescent molecularly imprinted paper chip and preparation methods and applications thereof. Background technique [0002] Carbon quantum dot (CDS) is a carbon-based zero-dimensional material, a nano cluster, approximate spherical type, and a structure and composition of a carbon quantum dot, and a structural and composition of a carbon quantum dot, and a structural and composition of a carbon quantum dot. Carbon quantum dots have strong absorption peaks in the ultraviolet region, and in the visible light regions have long traces, most absorbing peaks are concentrated in 260 to 320 nm, typically exhibiting optical characteristics such as fluorescence maximum emission wavelength, excitation wavelength dependence; A carbon quantum dot has a good water soluble under light, itself will emit bright fluorescence, and the o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 张忠秦潇潇马昕
Owner 陕西国环绿城环保科技有限公司
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