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Method of differentiation into mesenchymal stem cells through continuous subculture of dedifferentiated stem cells

A kind of stem cell and de-differentiation technology, which is applied in the field of differentiating de-differentiated stem cells into mesenchymal stem cells through continuous subculture, which can solve problems such as differentiation

Pending Publication Date: 2019-11-29
KYUNGPOOK NAT UNIV IND ACADEMIC COOP FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, due to the pluripotency of dedifferentiated stem cells, when differentiating dedifferentiated stem cells, there is a risk of differentiating into unwanted cells, so this is a problem that must be overcome in the field of dedifferentiated stem cell research and practical applications

Method used

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  • Method of differentiation into mesenchymal stem cells through continuous subculture of dedifferentiated stem cells
  • Method of differentiation into mesenchymal stem cells through continuous subculture of dedifferentiated stem cells
  • Method of differentiation into mesenchymal stem cells through continuous subculture of dedifferentiated stem cells

Examples

Experimental program
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Embodiment 1

[0069] Example 1. Preparation of dedifferentiated stem cells and the use of a medium for inducing differentiation to induce differentiation from dedifferentiated stem cells to mesenchymal stem cells

[0070] 1. Prepare dedifferentiated stem cells from horse adipose tissue.

[0071] Adipose tissue collected from 8-month-old horses was washed with Dulbecco's Phosphate-buffered saline (DPBS) (GeneDEPOT) and 70% ethanol (Duksan Pure Chemicals). The adipose tissue was cut into fine pieces using a cross cutter, added to phosphate buffered saline containing 0.2% type I collagenase (Worthington Biochemical), and decomposed in an incubator at 37°C for 10 minutes. The decomposed tissue was filtered with a 70 μm nylon cell strainer (SPL life sciences), the cell pellet was resuspended and washed with phosphate buffered saline to extract adult stem cells derived from horse adipose tissue. The stem cells derived from horse adipose tissue were extracted in a medium containing low glucose DMEM (D...

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Abstract

The present invention relates to a medium for inducing differentiation of dedifferentiated stem cells into mesenchymal stem cells, a method for preparing mesenchymal stem cells from dedifferentiated stem cells by using the same, and mesenchymal stem cells prepared by using the same. The mesenchymal stem cells prepared using the above medium and method can be differentiated into various target cells, and thus can be used usefully as a cell therapeutic agent for congenital and acquired musculoskeletal diseases and injuries.

Description

Technical field [0001] This application claims the priority and rights of Korean Patent Application No. 10-2017-0043781 filed on April 04, 2017, which is incorporated herein by reference for all purposes, as if fully explained herein. [0002] The present invention relates to a medium for inducing differentiation from dedifferentiated stem cells to mesenchymal stem cells, a method for preparing mesenchymal stem cells from dedifferentiated stem cells using the medium, and a mesenchymal prepared using the method stem cell. Background technique [0003] Dedifferentiated stem cells are pluripotency cells that can differentiate into three germ layers of ectoderm, mesoderm, and endoderm. This pluripotency is the greatest advantage of dedifferentiated stem cells, but in order to actually use dedifferentiated stem cells for clinical and drug screening, they must be differentiated into target cells. In addition, in order to reduce the risk of carcinogenesis, which is pointed out as the bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2500/34C12N2501/602C12N2501/603C12N2501/604C12N2501/606C12N2506/1384C12N2500/38C12N2500/25C12N2533/54C12N2501/165C12N5/0662C12N2506/025C12N2506/13C12N2501/33C12N2501/998
Inventor 郑圭植朴贤淑李恩周李顺礼金容得郑明珍李银美全述基
Owner KYUNGPOOK NAT UNIV IND ACADEMIC COOP FOUND
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