A pH-responsive and tumor-targeted anticancer drug carrier and preparation method thereof
An anticancer drug and tumor targeting technology, applied in the field of medicinal chemistry, can solve the problems of low drug loading rate and high only 19.2%, and achieve the effects of reducing drug resistance, obvious pH sensitivity, and easy operation.
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Embodiment 1
[0032] This embodiment provides a pH response and a tumor targeted dual drug anti-cancer carrier and a preparation method thereof, the preparation process figure 1 As shown, the specific steps are as follows:
[0033] (1) Preparation of polyteenaline-tyrosine graft (Pt): Weighing 0.36 G tyrosine to carry out 20 mL water for ultrasonic dispersion 20 min spare, and then take 0.8 g of polyteenine dissolution In 20 ml of water, 0.192 g of carbodiimide (EDC) was added to 0.115 g N-hydroxy succinimide (NHS) stirring reaction reaction for 40 min, and then added to the previously prepared tyrosine dispersion, stirred at 24 h. The unreacted tyrosine was removed by filtration, and the filtrate was dialyzed for three days, and then dried under 50 ° C. Grinding PT powder;
[0034] (2) Preparation of ethylenediamine - polyteenine - tyrosine (EPT): PT powder prepared by step (1) was dissolved in 30 ml of water, and 19.2 mg EDC and 14 mg NHS stirred reaction reaction 40 MIN, adding a drop of eth...
Embodiment 2
[0048] This embodiment provides a pH response and a tumor targeted dual drug anti-cancer carrier and a preparation method thereof, and the specific steps are as follows:
[0049] (1) Preparation of polyteenine-tyrosine graft (Pt): Weighing 0.108 G tyrosine to add 20 ml of water for ultrasonic dispersion 20 min spare, and separate 0.8 g of polyteenine dissolution In 50 ml of water, 0.038 g of carbon diimide (EDC) was added to 0.023 g N-hydroxy succinimide (NHS) stirring at 40 min, and then added to the previously prepared tyrosine dispersion, stirred at 24 h. The unreacted tyrosine was removed by filtration, and the filtrate was dialyzed for three days, and then dried under 50 ° C. Grinding PT powder;
[0050] (2) Preparation of ethylenediamine - polyteenine - tyrosine (EPT): PT powder prepared by step (1) was dissolved in water, and 3.8 mg EDC was added to 2.8 mg NHS stirring reaction 40 min. Heterdened ethylenediamine (EDA), stirred at a stirred reaction 24 h, three days by 2000 ...
Embodiment 3
[0055] This embodiment provides a pH response and a tumor targeted dual drug anti-cancer carrier and a preparation method thereof, and the specific steps are as follows:
[0056] (1) Preparation of polyteenine-tyrosine graft (Pt): Weighing 0.18 g of tyrosine to carry out 20 mL water for ultrasonic dispersion 20 min spare, and then take 0.8 g of polyteenine dissolution In 20 ml of water, 0.038 g of carbon diimide (EDC) was added to 0.023 g N-hydroxy succinimide (NHS) stirred by 40 min, and then the previously prepared tyrosine dispersion is added, and the reaction is stirred 24 h. The unreacted tyrosine was removed by filtration, and the filtrate was dialyzed for three days, and then dried under 50 ° C. Grinding PT powder;
[0057] (2) Preparation of ethylenediamine - polyteenine - tyrosine (EPT): PT powder prepared by step (1) was dissolved in water, and 3.8 mg EDC and 2.8 mg NHS stirred reaction reaction 40 MIN, adding a drop of ethylenediamine (EDA), stirring at 24 h, three days...
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Abstract
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