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A method for measuring the concentration of semi-licorice isoflavone b in blood plasma

A technology for isoflavones and plasma, applied in the field of determination of the concentration of semiglycyrrhizin isoflavone B in plasma, can solve the problems of the determination method of semiglycyrrhizin B in literature reports, and achieve the effects of high sensitivity, simple operation and fast method

Active Publication Date: 2022-06-24
ZHANGJIAGANG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no literature report on the in vivo determination method of semilicorice isoflavone B

Method used

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  • A method for measuring the concentration of semi-licorice isoflavone b in blood plasma
  • A method for measuring the concentration of semi-licorice isoflavone b in blood plasma
  • A method for measuring the concentration of semi-licorice isoflavone b in blood plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Determination of hemiglycyrrhizin B concentration in rat plasma

[0029] 1. Experimental materials and instruments

[0030] Hemi-licorice isoflavone B: isolated from dry roots and rhizomes of licorice; tinidazole (internal standard): purchased from China National Institute for Food and Drug Control, batch number: 100336-201704; test water: ultrapure water; methanol: chromatographically pure ( Merck Company).

[0031] API 4000LC-MS / MS triple quadrupole mass spectrometer with electrospray ionization (ESI), chromatography workstation: Analyst 1.6; Agilent 1200 high performance liquid chromatograph; AE240 electronic balance (Shanghai METTLER TOLEDO Co., Ltd. Company); MICRO-17R refrigerated centrifuge (Thermo Company, USA); WH-2 Micro Vortex Mixer (Shanghai Huxi Analytical Instrument Factory); Drict-Q5 Ultrapure Water Machine (Millipore Company, France).

[0032] 2. Liquid condition

[0033] 1. Liquid chromatography conditions

[0034] Chromatographic column:...

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Abstract

The invention discloses a method for measuring the concentration of semiglycyrrhizin isoflavone B in blood plasma. The liquid-mass spectrometry system is used for the measurement. Firstly, the sample to be tested is taken, and a certain amount of organic solvent protein precipitation agent is added to carry out protein precipitation. After pretreatment, the method is chromatographically Column separation and detection by a mass spectrometer, the method of the invention is fast, accurate, high in sensitivity and easy to operate, and is suitable for determining the concentration of the semiglycyrrhizin isoflavone B in blood plasma.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for determining the concentration of hemiglycyrrhizin B in plasma. Background technique [0002] Semilicoisoflavone B is a new flavonoid compound isolated from the dried root and rhizome of Glycyrrhiza uralensis Fisch. In vitro experiments have shown that hemi-licorice isoflavone B has significant anti-tumor, antioxidant, anti-inflammatory and other biological activities, and is a new drug source compound with good development prospects. Its structural formula is as follows: [0003] [0004] There is no literature report on the in vivo determination of hemi-licorice isoflavone B. SUMMARY OF THE INVENTION [0005] The technical problem to be solved by the present invention is to establish an accurate and high-efficiency method for measuring the concentration of hemi-glycyrrhizin Flavone B in plasma, and to carry out exploratory research on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72G01N30/88
CPCG01N30/02G01N30/06G01N30/7266G01N30/36G01N30/34G01N30/88G01N2030/027G01N2030/8822
Inventor 王建春蒋志涛柳春娣韩怡施晓卉
Owner ZHANGJIAGANG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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