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A kind of determination method of antioxidant content in high antioxidant fat emulsion injection

A determination method and oxidative technology, which can be used in measuring devices, instruments, scientific instruments, etc., can solve problems such as cumbersome operation, and achieve the effects of reducing interference, high efficiency, and low detection time cost.

Active Publication Date: 2022-07-12
湖北长联杜勒制药有限公司
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Problems solved by technology

[0003] Most of the oxidants currently used are DL-α-tocopherol, and the chemical name of DL-α-tocopherol is (±)-2,5,7,8-tetramethyl-2-(4′,8′,12′ -Trimethyltridecyl)-6-chromanol, also known as vitamin E alcohol, is a class of compounds that mainly exhibit vitamin E activity, because its vitamin activity was first tested in 1936 on fertility factors in rats It is identified in DL-α-tocopherol, so it is named "tocopherol". It exists in edible oils, fruits, vegetables and grains. It is a fat-soluble vitamin and an important antioxidant. The similar substances of DL-α-tocopherol include: d-α-tocopherol; d-α-tocopheryl ester; DL-α-tocopheryl ester; d-α-tocopheryl hydrogen succinate; DL-α-tocopheryl hydrogen succinate; β-tocopherol; γ-tocopherol; δ-tocopherol; tocopherol oil; all have the physiological activity of vitamin E, among which DL-α-tocopherol has the highest activity and the strongest antioxidant capacity, which has not been seen in the existing technology The analysis method is specially aimed at the detection of DL-α-tocopherol content in fat emulsion injection, and the existing reference method is cumbersome for the processing and detection of fat emulsion injection, and fails to achieve good liquid phase peak shape and good quality at the same time. The problem of separation, for this reason, the present invention proposes a kind of method for assaying antioxidant content in high antioxidant fat emulsion injection

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  • A kind of determination method of antioxidant content in high antioxidant fat emulsion injection
  • A kind of determination method of antioxidant content in high antioxidant fat emulsion injection
  • A kind of determination method of antioxidant content in high antioxidant fat emulsion injection

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Embodiment 1

[0024] Step 1: Sample pretreatment; details are as follows:

[0025] ①Preparation of the test solution: Weigh the sample, add n-hexane to ultrasonically dissolve and dilute to the mark; take an appropriate amount of ethanol to dilute to the mark, shake well, and filter with a 0.45 μm organic filter. The concentration of the test is about 5mg / mL;

[0026] ②Preparation of reference solution: Weigh DL-α-tocopherol reference substance, add ethanol to dissolve and dilute to the mark to prepare DL-α-tocopherol stock solution; The concentration of phenol is about 20 μg / mL;

[0027] Step 2: chromatographic conditions; the details are as follows:

[0028] ①chromatographic column: Symmetry C18, 250mm×4.6mm, 5μm; detection wavelength: 285nm; flow rate: 1.0ml / min;

[0029] ②Mobile phase: methanol-water (70:30); analysis time: 20min, isocratic elution; column temperature: 20℃; injection volume: 20μl;

[0030] Step 3: Determination; using the external standard method, inject the prepar...

Embodiment 2

[0032] Step 1: Sample pretreatment; details are as follows:

[0033] ①Preparation of the test solution: Weigh the sample, add n-hexane to ultrasonically dissolve and dilute to the mark; take an appropriate amount of ethanol to dilute to the mark, shake well, and filter with a 0.45 μm organic filter. The concentration of the test is about 5mg / mL;

[0034] ②Preparation of reference solution: Weigh DL-α-tocopherol reference substance, add ethanol to dissolve and dilute to the mark to prepare DL-α-tocopherol stock solution; The concentration of phenol is about 20 μg / mL;

[0035] Step 2: chromatographic conditions; the details are as follows:

[0036] ①chromatographic column: Symmetry C18, 250mm×4.6mm, 5μm; detection wavelength: 290nm; flow rate: 1.2ml / min;

[0037] ②Mobile phase: methanol-water (70:30); analysis time: 20min, isocratic elution; column temperature: 30℃; injection volume: 20μl;

[0038] Step 3: Determination; using the external standard method, inject the prepar...

Embodiment 3

[0040] Step 1: Sample pretreatment; details are as follows:

[0041] ①Preparation of the test solution: Weigh the sample, add n-hexane to ultrasonically dissolve and dilute to the mark; take an appropriate amount of ethanol to dilute to the mark, shake well, and filter with a 0.45 μm organic filter. The concentration of the test is about 5mg / mL;

[0042] ②Preparation of reference solution: Weigh DL-α-tocopherol reference substance, add ethanol to dissolve and dilute to the mark to prepare DL-α-tocopherol stock solution; The concentration of phenol is about 20 μg / mL;

[0043] Step 2: chromatographic conditions; the details are as follows:

[0044] ①chromatographic column: Symmetry C18, 250mm×4.6mm, 5μm; detection wavelength: 280nm; flow rate: 0.8ml / min;

[0045] ②Mobile phase: methanol-water (70:30); analysis time: 20min, isocratic elution; column temperature: 20℃; injection volume: 20μl;

[0046]Step 3: Determination; using the external standard method, inject the prepare...

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Abstract

The invention provides a method for determining the content of antioxidants in a high antioxidant fat emulsion injection. The steps of the determination method are as follows: sample pretreatment, chromatographic conditions, and determination; a high antioxidant fat emulsion injection of the invention Compared with the previous technology, the method for determining the content of antioxidants in the medium is compared with the prior art. In the pretreatment process, n-hexane is used to dissolve the sample, and further dilution with ethanol is used to reduce interference. The operation is simple and convenient. high.

Description

technical field [0001] The invention belongs to the field of pharmaceutical analysis, and particularly relates to a method for determining the content of antioxidants in a high antioxidant fat emulsion injection. Background technique [0002] Fat emulsion injection is a kind of parenteral nutritional supplement that provides energy and essential fatty acids with a wide range of clinical applications. Oil mixture) is the oil phase, supplemented by lecithin as an emulsifier, and glycerol as an osmotic regulator, which is prepared by appropriate emulsification technology. During the preparation process, the fat emulsion is easily affected by factors such as oxygen, light and temperature. Oxidative deterioration occurs. In the process of oxidation, the oil for injection will produce peroxides, which will be decomposed into small molecular complex compounds such as aldehydes, ketones, acids, etc., which can destroy the normal physiological function of human cell membranes and pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/89
CPCG01N30/06G01N30/89G01N2030/146G01N2030/047
Inventor 郭文胜
Owner 湖北长联杜勒制药有限公司
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