Brevibacillus laterosporus, antibacteriallipopeptide and application thereof to agriculture and food
A Brevibacillus and antibacterial lipopeptide technology, applied in the biological field, can solve the problems of unsatisfactory bacteriostatic effect, high price, low yield, etc., and achieve the effect of good inhibition and killing effect, prolonging shelf life and wide antibacterial spectrum.
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[0048] 1. Screening and identification of Brevibacillus spp.
[0049] (1) Sieve of Brevibacillus sporogenes: After the soil sample was suspended, it was bathed in water at 80°C for 10 minutes, and diluted tenfold to obtain 10 -2 , 10 -3 , 10 -4 For three concentrations, take 0.1 mL of each of the above three concentrations, spread on LB plates, and incubate at 37°C for 24 hours, pick single colonies with different colony shapes, inoculate them into LB liquid medium at 30°C, and incubate at 180rpm for 24 hours. The fermentation supernatant was taken to select Escherichia coli and Staphylococcus aureus for antibacterial experiments. The strains with antibacterial effect were confirmed in the next step.
[0050] (2) Identification of Brevibacillus spp.: Morphological identification and 16S rRNA sequence alignment.
[0051] Morphological identification includes Gram staining and spore staining, and morphological identification is carried out on the screened strains with antiba...
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