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A kind of Brevibacillus spp., antibacterial lipopeptide and its application in agriculture and food

A technology of Bacillus brevius and antibacterial lipopeptide, applied in the biological field, can solve the problems of high price, low yield, unstable antibacterial performance, etc., and achieve the effect of extending shelf life, safe source, and eliminating use

Active Publication Date: 2022-02-01
NANJING AGRICULTURAL UNIVERSITY +1
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  • Claims
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AI Technical Summary

Problems solved by technology

[0005] Although there are many studies on microbial natural preservatives from different sources such as lactic acid bacteria bacteriocins and antibacterial lipopeptides at home and abroad, there are few practical applications in the food industry. Unsatisfactory, unstable antibacterial performance, especially the high price, low yield and uncertain safety, it is still difficult to replace the role and status of chemical preservatives

Method used

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  • A kind of Brevibacillus spp., antibacterial lipopeptide and its application in agriculture and food
  • A kind of Brevibacillus spp., antibacterial lipopeptide and its application in agriculture and food
  • A kind of Brevibacillus spp., antibacterial lipopeptide and its application in agriculture and food

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Embodiment 1

[0048] 1. Screening and identification of Brevibacillus spp.

[0049] (1) Sieve of Brevibacillus sporogenes: After the soil sample was suspended, it was bathed in water at 80°C for 10 minutes, and diluted tenfold to obtain 10 -2 , 10 -3 , 10 -4 For three concentrations, take 0.1 mL of each of the above three concentrations, spread on LB plates, and incubate at 37°C for 24 hours, pick single colonies with different colony shapes, inoculate them into LB liquid medium at 30°C, and incubate at 180rpm for 24 hours. The fermentation supernatant was taken to select Escherichia coli and Staphylococcus aureus for antibacterial experiments. The strains with antibacterial effect were confirmed in the next step.

[0050] (2) Identification of Brevibacillus spp.: Morphological identification and 16S rRNA sequence alignment.

[0051] Morphological identification includes Gram staining and spore staining, and morphological identification is carried out on the screened strains with antiba...

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Abstract

The invention discloses a Brevibacillus lateralsporosa, an antibacterial lipopeptide and its application in agriculture and food, and belongs to the field of biotechnology. Among them, Brevibacillus spp. was isolated from Nanjing farmland; the Bacillus spp. was cultured in LB liquid medium, and it could be fermented to produce antibacterial lipopeptide, wherein the antibacterial lipopeptide produced was composed of two amino acid sequences. Two kinds of antibacterial lipopeptides are obtained through three separations, purifications and identifications. After determination, the antibacterial lipopeptide has a broad antibacterial spectrum and good thermal stability and pH stability, and can be widely used in the fields of food and agriculture.

Description

technical field [0001] The invention relates to a Brevibacillus lateralosporus, an antibacterial lipopeptide and its application in agriculture and food, and belongs to the field of biotechnology. Background technique [0002] In the food industry, the anticorrosion and preservation of food is always an important problem to be solved urgently. According to FDA statistics, about 10%-20% of the food in the world is lost every year due to various spoilage, and microorganisms play a decisive role in the process of food spoilage. Aiming at the problem of food spoilage caused by microorganisms, we adopt different methods to control the microorganisms in food. However, the commonly used high-temperature sterilization method will have adverse effects on the texture, flavor and nutrition of food, and the method of adding chemical preservatives will have an impact on human health. Natural food preservatives are still one of the important measures for food preservation because they a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C07K7/06C07K1/14A23L3/3526A61K38/10A61P31/04C12R1/01
CPCC07K7/06A23L3/3526A61P31/04A23V2002/00A61K38/00C12R2001/01C12N1/205A23V2200/10A23V2250/55
Inventor 陆兆新吴宇博李金良吕凤霞别小妹周立邦
Owner NANJING AGRICULTURAL UNIVERSITY
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