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Method for comparing and distinguishing Wen country iron stick yam and other varieties of yam based on 1HNMR metabonomics technology

A technology of iron stick yam and metabolomics, applied in the field of plant metabolomics, can solve the problems such as the difficulty of effective identification, supervision and quality management of yam varieties.

Active Publication Date: 2019-12-20
河南省人口和计划生育科学技术研究院
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Problems solved by technology

Wu Xujin et al. (Chinese invention patent application 201711170036.4) used nuclear magnetic resonance technology to identify the compounds in iron yam with skin and iron yam without skin. The results showed that taurine, glycine, 1,5- The contents of dimethyl citrate, sucrose, fumaric acid, and yam are relatively high, while the contents of dehydroepiandrosterone, isoleucine, valine, and threonine are relatively low. This method only compares peeled The difference from the active ingredients of the unpeeled iron rod yam itself makes it impossible to effectively identify the yam species, which brings difficulties to the supervision and quality management of the agricultural and food industries

Method used

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  • Method for comparing and distinguishing Wen country iron stick yam and other varieties of yam based on 1HNMR metabonomics technology
  • Method for comparing and distinguishing Wen country iron stick yam and other varieties of yam based on 1HNMR metabonomics technology
  • Method for comparing and distinguishing Wen country iron stick yam and other varieties of yam based on 1HNMR metabonomics technology

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Embodiment 1

[0066] a based on 1 HNMR metabolomics technology compares the method of planting iron stick yam and characteristic metabolites of other varieties of yam in Wen County, including the following steps:

[0067] 1. Preparation of yam samples: Clean the iron stick yam planted in Wenxian County, Chenji iron stick yam, Yongji iron stick yam, Yuncheng iron stick yam, Xiaobaizui yam, chicken skin rough yam, purple yam and Xishi yam, Dry in the air, cut into 2-3mm thin slices and dry at 50°C, crush for later use. Eight replicates of each yam sample were prepared.

[0068] 2. Extraction of ingredients in yam: Weigh 50 mg of yam powder prepared in the above steps and place them in a 1.5 mL centrifuge tube, add 0.8 mL of a mixed solvent of deuterated methanol and heavy water (CD 3 OD / D 2 O=1 / 1, v / v), vortex for 5 minutes, centrifuge at 10000g for 5 minutes, absorb 600 μL of supernatant and place in a 5mm sample tube;

[0069] 3. 1 H NMR test: put the 5mm sample tube in the Agilent 400...

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Abstract

The invention relates to the technical field of plant metabolomics, in particular to a method for comparing and distinguishing Wen country iron stick yam and other varieties of yam based on 1H NMR metabonomics technology. The method provided by the invention adopts H NMR-based metabolomics technology and uses principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to analyze the metabolites in the Wen country iron stick yam and the iron stick yam planted in other regions, and finally determines the difference between the metabolites between the Wen country planted iron stick yam and the iron stick yam planted in other regions, thereby providing a scientific basis for variety identification, true and false identification and the like of the Wen country iron stick yam as famous-region drug.

Description

technical field [0001] The invention relates to the technical field of plant metabolomics, in particular to a 1 H NMR metabolomics technology to compare and distinguish Wenxian Tiegon yam from other varieties of yam. Background technique [0002] The current research literature on the composition of Tiegon yam and other varieties of yam is limited to moisture, total ash, nitrogen content, reducing sugar, mucopolysaccharide, starch, crude fat, crude fiber, amino acids, etc. Effective identification of yam origin and other yam varieties. Zhang Min et al. (Food Engineering, No. 4, 2014) identified the different chemical components in raw yam decoction pieces, bran-fried yam and used yam, mainly to understand the influence of processing on yam components and the differences between medicinal materials and food materials. The origin and variety of yam were not identified. Wu Xujin et al. (Chinese invention patent application 201711170036.4) used nuclear magnetic resonance tech...

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Application Information

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IPC IPC(8): G01N24/08
CPCG01N24/087Y02A90/30
Inventor 王强王艳丽吴宗胤祁少可王晓慧张超楠郝戈芳李乐瑶刘新新
Owner 河南省人口和计划生育科学技术研究院
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