Analysis method for rapidly screening pesticide and metabolite residue in tea

Abstract

The invention relates to an analysis method for rapidly screening pesticide and metabolite residue in tea. The analysis method comprises the steps of analyzing by Sin-QuEChER dispersive solid-phase extraction combined with an ultrahigh performance liquid chromatography-quadrupole-static field rail well high-resolution mass spectrum method; extracting a sample with 0.1% formic acid acetonitrile solution; performing salt precipitation oscillation and centrifugation; further purifying with Sin-QuEChERS Nano dispersive solid-phase extraction column; performing separation with Thermo Accucore aQ (2.1 millimeter by 150 millimeter and 2.6 micrometers) chromatographic column, and performing gradient elution with methyl alcohol, 0.1% methyl alcohol and 5mmol / L ammonium formate solution; and analyzing in a Full MS / ddMS2 scanning mode under a positive ion mode. The method is simple, convenient, rapid, efficient and accurate in handling process and can be used for rapidly screening multiple pesticide and metabolins in the tea.

Description

Technical field [0001] The invention belongs to the field of analysis and detection, and specifically relates to an analysis method for rapidly screening pesticide and metabolite residues in tea. It adopts Sin-QuEChER dispersed solid phase extraction combined with ultra-high performance liquid chromatography-quadrupole-electrostatic field orbital high Analysis by resolution mass spectrometry. Background technique [0002] Tea is known as one of the three major beverages in the world because of its anti-cancer, anti-oxidation, and fat-reducing health effects and its unique taste. In order to control the effects of pests and weeds, long-term use of pesticides will lead to pesticide residues in the finished tea. In view of the widespread consumption of tea and the potential health risks of pesticides to humans, many countries and organizations have stipulated maximum residue limits (MRLs) for pesticide residues in tea. For example, the relevant standards of the European Union and ...

AI Technical Summary

Problems solved by technology

[0004] The organic acids, alkaloids and pigments in tea leaves make tea pretreatment difficult with low purification efficiency, large matrix effect, and cumbersome steps.

Saito-Shida S et al reported the analysis of 146 kinds of pesticide residues in tea by liquid chromatography-high resolution mass spectrometry. The samples need to be extracted repeatedly, concentrated and blown with nitrogen to make up the volume, and the operation is cumbersome.

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