Combined fluorescence and absorption detector for on-column detection after capillary separation techniques

Abstract

A system and method for performing UV LED-based absorption detection for capillary liquid chromatography for detecting and quantifying compounds in a liquid, wherein a simplified system eliminates theneed for a beam splitter and a reference cell by using a stable UV source, and power requirements are reduced, resulting in a portable and substantially smaller system with relatively low detection limits.

Description

Background technique [0001] Related technical description: Many techniques for separating mixtures of compounds rely on the movement of compounds through channels or tubes called columns, where the compounds appear as a series of bands at the end of the column. The detection and identification of compounds in bands upon exiting the separation column is a critical step in a successful separation technique. [0002] An efficient detection scheme should not degrade the separation already occurring in the separation column. Unfortunately, adding flow cells or other accessories to a separation column (especially for columns with capillary dimensions) often degrades the separation. Therefore, it is more desirable to perform detection directly on the separation column. [0003] Absorption spectrophotometry and fluorescence spectrophotometry are two spectroscopic techniques that are effectively used for condensed phase separation detection. An advantage of absorption spectrophotom...

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Problems solved by technology

Therefore, low-pass filtering is clearly not sufficient to effectively remove high-frequency noise from the detection system

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