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Method for cultivating watermelon variety with high resistance to fusarium wilt

A technology of high resistance to Fusarium wilt and watermelon varieties, applied in the agricultural field, can solve the problems of long seedling time, limitation, and inability to solve the problems of seeds carrying pathogenic bacteria, and achieve the effects of high efficiency, short cycle and reliable breeding materials

Pending Publication Date: 2019-12-31
黑龙江省农业科学院园艺分院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because pathogenic bacteria can survive in the soil for more than 6 years, crop rotation and intercropping are limited; grafting seedlings consumes more manpower, material and financial resources, takes longer to raise seedlings and affects the quality of watermelons, and cannot solve the problem of pathogenic bacteria carried by seeds at the same time. The incidence of root rot and stem rot will increase significantly when watermelon is grafted

Method used

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  • Method for cultivating watermelon variety with high resistance to fusarium wilt
  • Method for cultivating watermelon variety with high resistance to fusarium wilt
  • Method for cultivating watermelon variety with high resistance to fusarium wilt

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Effect test

Embodiment 1

[0032] 1. Parent's name and source

[0033] Calhoun Gray: is a typical high-resistance to Fusarium wilt physiological race 1 American type watermelon material.

[0034] K10197 is a stable inbred line bred in 2014 from the first cross of Korean watermelon variety KW10-1 introduced in 2012 and selfed for 8 generations. Fresh-eating hybrids. The growth period is about 90 days, the plant growth is strong, the fruit development period is about 30 days, the fruit has an oval flower skin, a single fruit weighs about 10 kg, and the flesh is red. The peel has good toughness, crack resistance and transportation resistance. The fruit profile is uniform, the edible rate of the fruit is high, the meat is crisp, the taste is sweet, and the flavor is good.

[0035] The seedling age is about 25 days. Climbing cultivation, plant spacing 60 cm, row spacing 210 cm. 30,000 kg of farmyard manure, 270 kg of diammonium, 270 kg of potassium sulfate, and 75 kg of cake fertilizer (cooked soybeans) per he...

Embodiment 2

[0075] Beginning in 2015, the research team used Calhoun Gray as the source of resistance to Fusarium wilt physiological race 1, and simultaneously prepared multiple pairs of backcross breeding combinations, including K10197, and implemented the innovative work of double-selection of anti-fusarium wilt materials in separate nurseries, eliminating the third generation of Hainan During backcrossing and selfing, INDELL molecular markers and high incidence nurseries for fusarium wilt were used for targeted selection of fusarium wilt resistance.

[0076] In July 2015, BW88 and BWB051 were used as recurrent parents, and Calhoun Gray was used to prepare hybrid combinations. According to the method of Example 1, high resistance materials BW88FR(122) and BWB051 FR- were bred through 4 generations of backcross and 4 generations of self-crossing. 1(282). In 2019, after seedling stage inoculation, the disease index of 122 was 2.98 and the diseased plant rate was 4.99%; the disease index of 2...

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Abstract

The invention discloses a method for cultivating a watermelon variety with high resistance to fusarium wilt. The method comprises the following steps: taking a breeding material A of a physiological race 1 with high resistance to fusarium wilt of watermelons as a donor parent, taking a breeding material B of a disease-sensitive high-quality material as a recurrent parent for hybridization, continuously backcrossing for 4 generations, then carrying out selfing, and selecting homozygous disease-resistant genotype plants for keeping selfing until homozygous disease-resistant genotype inbred linesare obtained. DNA is extracted from each backcross generation and self-bred offspring at one-leaf and one-core stage of a seedling, screening is carried out by using an Indell-fon1 marker, hybrid disease-resistant genotype plants are selected and planted in a fusarium wilt disease nursery for disease nursery screening, and single plant seed collection of plants with good growth state and agronomic characters close to recurrent parents is selected. According to the method disclosed by the invention, 'nursery-separation double-selection' is adopted, double screening is carried out by a method of combining molecular markers with disease nursery screening, the breeding material obtained by the method is reliable, the incidence rate of the fusarium wilt of the breeding material can be controlled at 20% or below, and accurate breeding of the watermelon variety resistant to the fusarium wilt is realized.

Description

Technical field [0001] The invention relates to a method for cultivating watermelon varieties, in particular to a method for cultivating watermelon varieties with high resistance to fusarium wilt. The invention belongs to the field of agricultural technology. Background technique [0002] Watermelon is one of the top ten fruits in the world, and has the reputation of "the king of summer fruits". According to FAO statistics, in 2013, the global watermelon harvest area was about 3.48 million hectares, ranking seventh among the fruits, with an output of about 109.27 million tons. Ranked first in fruit. However, due to the watermelon wilt disease caused by Fusarium oxysporum, the production of watermelons has been greatly reduced, and this disease has become one of the most serious diseases that harm the watermelon industry. [0003] Watermelon fusarium wilt is commonly known as "dead seedling disease". In the early stage of the disease, the leaves on the stems of the diseased plant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/6858
CPCA01H1/02A01H1/04C12Q1/6858C12Q2531/113C12Q2565/125
Inventor 王喜庆闫闻贾云鹤付永凯尤海波
Owner 黑龙江省农业科学院园艺分院
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