A kind of all-liquid phase organ chip and preparation method thereof

An organ chip and liquid phase technology, applied in the field of biochip and its preparation, can solve the problems of inability to simulate the dynamic changes of human tissue, complex system, difficult to change the structure, etc. Effect

Active Publication Date: 2022-07-29
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, some tests, such as cell sequencing, need to take samples from the inside of the chip, so the organ chip needs to integrate sampling flow channels and interfaces, which makes the whole system very complicated, which greatly restricts the development of the organ chip
In addition, the current organ chip is difficult to change its structure after construction, which makes it unable to simulate the dynamic changes of human tissue, and in some cases cannot achieve bionic functions well.

Method used

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  • A kind of all-liquid phase organ chip and preparation method thereof
  • A kind of all-liquid phase organ chip and preparation method thereof
  • A kind of all-liquid phase organ chip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The process of preparing an all-liquid phase organ chip in this embodiment is as follows: figure 1 shown, including the following steps:

[0035] (1) Preparation of surface-modified glass with double bonds

[0036] Add 30 μL of 3-(methacryloyloxy)propyltrimethoxysilane solution to the overlapping gap between the two pretreated hydroxy glasses, so that the solution can completely infiltrate the surface of the overlapping glass while ensuring no bubbles, and react for 1 h , respectively, washed several times with water and ethanol, and dried to obtain a glass slide with surface modified double bonds;

[0037] The mass concentration of 3-(methacryloyloxy)propyltrimethoxysilane in the 3-(methacryloyloxy)propyltrimethoxysilane solution is 20 wt %, and the solvent is ethanol.

[0038] (2) Preparation of polymer layer porous membrane

[0039] Prepare the prepolymer mixture: The prepolymer mixture solution includes the following components by volume percentage: 12vol% HEMA (...

Embodiment 2

[0051] (1) Preparation of surface-modified double bond substrates

[0052] This step is basically the same as Example 1, except that the mass concentration of 3-(methacryloyloxy)propyltrimethoxysilane in the 3-(methacryloyloxy)propyltrimethoxysilane solution is 10wt%, The solvent is ethanol.

[0053] Wherein, the substrate is plastic, and the plastic substrate is pretreated with oxygen plasma to make it hydrophilic, and then the same steps as in Example 1 are used to modify the substrate.

[0054] (2) Preparation of polymer layer porous membrane

[0055] The polymer layer porous film prepared in this step is basically the same as that in Example 1, except that the prepolymer mixture solution includes 15vol% HEMA (monomer), 6vol% EDMA (crosslinking agent), 50vol% cyclohexanol ( porogen), 29vol% n-decanol (porogen), and benzoin dimethyl ether (DMPA) is used as the photoinitiator, and the addition of the photoinitiator accounts for the concentration of the mixture to be 0.5mg / m...

Embodiment 3

[0065] (1) Preparation of surface-modified glass with double bonds

[0066] This step is basically the same as Example 1, except that the mass concentration of 3-(methacryloyloxy)propyltrimethoxysilane in the 3-(methacryloyloxy)propyltrimethoxysilane solution is 25wt%, The solvent is ethanol.

[0067] (2) Preparation of polymer layer porous membrane

[0068] The polymer layer porous film prepared in this step is basically the same as that in Example 1, except that the prepolymer mixture solution includes 16vol% HEMA (monomer), 4vol% EDMA (crosslinking agent), 40vol% cyclohexanol ( porogen), 40vol% n-decanol (porogen), and benzoin dimethyl ether (DMPA) is used as the photoinitiator, and the addition of the photoinitiator accounts for 4mg / mL of the mixture;

[0069] (3) Preparation of hydrophobic polymer layer porous membrane

[0070] In a fume hood environment, 40 mL of dichloromethane (DCM), 80 μL of 4-pentynoic acid and 100 μL of N,N′-diisopropylcarbodiimide (DIC) were add...

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Abstract

The invention discloses an all-liquid phase organ chip and a preparation method thereof. The all-liquid phase organ chip comprises a super-hydrophobic porous membrane with a hydrophilic channel, the porous membrane is sealed in an oil phase, and a hydrophilic channel is provided on the hydrophilic channel to communicate with it. The fluid inlet and outlet are obtained; the hydrophobic polymer layer porous membrane is obtained by placing the polymer layer porous membrane in the functional modification solution; the hydrophilic pattern treatment is performed on the hydrophobic polymer layer porous membrane; The surface of the porous membrane is subjected to super-hydrophobic treatment on the surface of the polymer; the hydrophilic channel on the surface of the porous membrane is then wetted, placed in the oil phase to seal, and an inlet and an outlet connected to the hydrophilic channel are arranged. The preparation conditions of the invention are mild, the patterned culture of cells can be constructed on the prepared substrate to realize the construction of an all-liquid phase organ chip, the in-situ detection and sampling of the microenvironment in the organ chip can be realized, and the fluid can be collected as required. The functional and geometric layout of the installation is spatially edited.

Description

technical field [0001] The invention relates to the field of biological chips and preparation methods thereof, in particular to an all-liquid phase organ chip and preparation method thereof. Background technique [0002] New drug research and development is a time-consuming and costly industry. Currently, the average development cost of a new drug is about $1 billion, and it takes an average of 10 years, making the development of new drugs extremely difficult. At present, 90% of the drugs that have passed the cell experiments and animal experiments in the drug screening will be rejected in the clinical testing stage. The reason is mainly because the cell experiments and animal models are quite different from the human model, so the development is closer to the human body. The model's drug testing platform is extremely important. In recent years, with the rapid development of biotechnology and information technology, organ-on-a-chip has become a unique and dynamic emerging ...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12M3/06C12M1/00C08F220/20C08F222/14C08J9/26
CPCC12M21/08C12M25/04C12M23/16C08F220/20C08J9/26
Inventor顾忠泽杜鑫李玉雯
OwnerSOUTHEAST UNIV