A kind of Pseudomonas balearicum and its application in treating landfill leachate membrane concentrate

Abstract

The invention discloses a Pseudomonas balearica named as Pseudomonas balearica EBT-1, which was preserved in the China Center for Type Culture Collection on September 17, 2019, with a preservation number for CCTCC M 2019730. The invention also discloses the application of the above-mentioned Pseudomonas balearica in treating landfill leachate membrane concentrate. The present invention obtains high-salt-resistant Pseudomonas balearica (Pseudomonas balearica) EBT‑1 after gradually acclimating landfill leachate membrane concentrate, and the Pseudomonas balearica (Pseudomonas balearica) EBT‑1 1 It can effectively remove the total nitrogen content in the landfill leachate membrane concentrate.

Description

technical field

[0001] The invention relates to a kind of Pseudomonas balearicus, and also relates to the application of the pseudomonas balearicus in treating landfill leachate membrane concentrate, which belongs to the field of sewage treatment. Background technique

[0002] Landfill leachate is a kind of high-concentration organic wastewater with complex components, which has the characteristics of poor biodegradability, high concentration of ammonia nitrogen, and rich content of toxic and harmful substances. With the increasingly stringent environmental water quality control standards, especially in October 2009, the "Technical Specifications for Leachate Treatment Engineering of Domestic Waste Landfill Sites (Draft for Comment)" was promulgated, which proposed that advanced treatment should be based on membrane treatment technology, and further The standardization trend of the membrane process has been determined. As the mainstream process, the advanced treatment proces...

Examples

Embodiment 1

[0029] Example 1: Isolation and Identification of Bacterial Strains

[0030] The strain EBT-1 is an aerobic denitrifying bacterium with denitrifying activity screened from the activated sludge of the landfill leachate sewage treatment system of the waste incineration plant.

[0031] The separation and screening method is: take out the activated sludge from the landfill leachate (high salt) sewage treatment biochemical system; inoculate 5g of the activated sludge into the DM inorganic salt medium (the salinity in the medium is 100mg / L) of the nitrate nitrogen concentration >3%), cultured on a shaker at a temperature of 35°C and a rotating speed of 150rpm for 1 day to obtain the first bacterial solution; the first bacterial solution was inoculated to a nitrate nitrogen concentration of 200 mg at an inoculum size of 5% (volume fraction) In the DM inorganic salt culture medium (salinity>3% in the culture medium) of 1 / L, at temperature 35 ℃, the shaker that rotating speed is 150rpm...

Embodiment 2

[0037] Embodiment 2: the expanded cultivation of Pseudomonas balearica (Pseudomonas balearica) EBT-1 of the present invention:

[0038] The medium used in the expanded culture includes the following components: 10 g of corn steep liquor (dry powder), 5 g of yeast extract, 10 g of sodium chloride, 2.5 g of anhydrous sodium acetate, 1 g of dipotassium hydrogen phosphate and 0.03 g of magnesium sulfate per liter of water , the initial pH of the medium was 6.5.

[0039] The production process of the culture medium is as follows: dissolve 10 g of corn steep liquor (dry powder), 5 g of yeast extract, 10 g of sodium chloride, 2.5 g of anhydrous sodium acetate, 1 g of dipotassium hydrogen phosphate, and 0.03 g of magnesium sulfate in 1000 g of water, and adjust it with alkali. The pH was 6.5, and it was sterilized at 121° C. for 20 minutes to obtain a culture medium.

[0040] Pseudomonas balearica (Pseudomonas balearica) EBT-1 bacteria mother solution is inoculated in the culture med...

Embodiment 3

[0042] Embodiment 3: the investigation of salinity adaptability of Pseudomonas balearica (Pseudomonas balearica) EBT-1 of the present invention

[0043] The Pseudomonas balearica (Pseudomonas balearica) EBT-1 expansion culture solution obtained in Example 2 was centrifuged at 6000 rpm for 5 minutes, and the supernatant was removed to obtain activated cell solids. Set four salinity gradients of 0%, 3%, 7%, and 10%. Per liter of salinity determination distribution water contains the following components in mass fraction: glucose 6.1g, KNO 3 6.07g, KH 2 PO 4 1.5g, K 2 HPO 4 ·3H 2 O 6.0g, MgSO 4 ·7H 2 O 1.0g, NaCl adjusted according to desired salinity. The centrifuged bacterial solid was inoculated into the water for salinity measurement according to the inoculum size of 0.4%, and was shaken at 150 rpm at 30 °C in a 250 mL blue cap bottle.

[0044] Detect the change of total nitrogen during the reaction process under different salinity conditions, the results are shown...

technical field

[0001] The invention relates to a kind of Pseudomonas balearicus, and also relates to the application of the pseudomonas balearicus in treating landfill leachate membrane concentrate, which belongs to the field of sewage treatment. Background technique

[0002] Landfill leachate is a kind of high-concentration organic wastewater with complex components, which has the characteristics of poor biodegradability, high concentration of ammonia nitrogen, and rich content of toxic and harmful substances. With the increasingly stringent environmental water quality control standards, especially in October 2009, the "Technical Specifications for Leachate Treatment Engineering of Domestic Waste Landfill Sites (Draft for Comment)" was promulgated, which proposed that advanced treatment should be based on membrane treatment technology, and further The standardization trend of the membrane process has been determined. As the mainstream process, the advanced treatment proces...

Examples

Embodiment 1

[0029] Example 1: Isolation and Identification of Bacterial Strains

[0030] The strain EBT-1 is an aerobic denitrifying bacterium with denitrifying activity screened from the activated sludge of the landfill leachate sewage treatment system of the waste incineration plant.

[0031] The separation and screening method is: take out the activated sludge from the landfill leachate (high salt) sewage treatment biochemical system; inoculate 5g of the activated sludge into the DM inorganic salt medium (the salinity in the medium is 100mg / L) of the nitrate nitrogen concentration >3%), cultured on a shaker at a temperature of 35°C and a rotating speed of 150rpm for 1 day to obtain the first bacterial solution; the first bacterial solution was inoculated to a nitrate nitrogen concentration of 200 mg at an inoculum size of 5% (volume fraction) In the DM inorganic salt culture medium (salinity>3% in the culture medium) of 1 / L, at temperature 35 ℃, the shaker that rotating speed is 150rpm...

Embodiment 2

[0037] Embodiment 2: the expanded cultivation of Pseudomonas balearica (Pseudomonas balearica) EBT-1 of the present invention:

[0038] The medium used in the expanded culture includes the following components: 10 g of corn steep liquor (dry powder), 5 g of yeast extract, 10 g of sodium chloride, 2.5 g of anhydrous sodium acetate, 1 g of dipotassium hydrogen phosphate and 0.03 g of magnesium sulfate per liter of water , the initial pH of the medium was 6.5.

[0039] The production process of the culture medium is as follows: dissolve 10 g of corn steep liquor (dry powder), 5 g of yeast extract, 10 g of sodium chloride, 2.5 g of anhydrous sodium acetate, 1 g of dipotassium hydrogen phosphate, and 0.03 g of magnesium sulfate in 1000 g of water, and adjust it with alkali. The pH was 6.5, and it was sterilized at 121° C. for 20 minutes to obtain a culture medium.

[0040] Pseudomonas balearica (Pseudomonas balearica) EBT-1 bacteria mother solution is inoculated in the culture med...

Embodiment 3

[0042] Embodiment 3: the investigation of salinity adaptability of Pseudomonas balearica (Pseudomonas balearica) EBT-1 of the present invention

[0043] The Pseudomonas balearica (Pseudomonas balearica) EBT-1 expansion culture solution obtained in Example 2 was centrifuged at 6000 rpm for 5 minutes, and the supernatant was removed to obtain activated cell solids. Set four salinity gradients of 0%, 3%, 7%, and 10%. Per liter of salinity determination distribution water contains the following components in mass fraction: glucose 6.1g, KNO 3 6.07g, KH 2 PO 4 1.5g, K 2 HPO 4 ·3H 2 O 6.0g, MgSO 4 ·7H 2 O 1.0g, NaCl adjusted according to desired salinity. The centrifuged bacterial solid was inoculated into the water for salinity measurement according to the inoculum size of 0.4%, and was shaken at 150 rpm at 30 °C in a 250 mL blue cap bottle.

[0044] Detect the change of total nitrogen during the reaction process under different salinity conditions, the results are shown...

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