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Method for removing egg membrane during grouper hatching

A technology of grouper and egg membranes, applied in fish farming, climate change adaptation, application, etc., can solve the problems of destroying fish eggs, separating and dissolving egg membranes, reducing the survival rate of fish eggs, etc., to prevent oxidation reactions , increase the thickness, not easy to fall off

Active Publication Date: 2021-11-12
HAINAN CHENHAI AQUATIC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Grouper roe and fish roe with egg membrane have a thick egg membrane, and the outermost end has a complex multi-layered protein shell with a certain thickness, and the egg membrane and the cytoplasmic membrane are closely attached, and it is difficult to separate and dissolve the egg membrane. Research on the structure of grouper egg membrane brings many inconveniences
At present, the use of physical film removal for fish egg membrane removal will destroy fish eggs, and the use of chemical agents will reduce the survival rate of fish eggs, and the separation effect on grouper egg membranes is not good.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for removing egg membranes during grouper hatching: comprising the following steps:

[0017] S1. Hypotonic treatment: Add phosphate buffered saline solution to the hatching tank for rinsing. The volume ratio of phosphate buffered saline solution to egg cells is 30:17. After rinsing twice, soak the egg cells, mix and let stand for 6 minutes at 24°C;

[0018] S2. Stir and debond: Put the fish eggs into the mud and stir for 10 minutes to debond and then take it out. The mud and water are mixed and stirred evenly in a ratio of 1:5. After passing through an 80-mesh sieve, put them into the hatching tank and wash them with water. , the water velocity is 1m / s, and the water pressure is 0.03MPa;

[0019] S3. Hatching and stripping: add hatching medium, adjust pH value to 5, water temperature 28°C, hatch for 20h, then add hatching medium, adjust pH value to 5.7, water temperature 28°C, cultivate for 50h, the hatching medium is quality Butylated hydroxyanisole, dibutyl ...

Embodiment 2

[0022] A method for removing egg membranes during grouper hatching: comprising the following steps:

[0023] S1. Hypotonic treatment: Add phosphate buffered saline solution to the hatching tank for rinsing. The volume ratio of phosphate buffered saline solution to egg cells is 35:20. After rinsing 4 times, soak the egg cells, mix and let stand for 15 minutes at 30°C;

[0024] S2. Stir and debond: Put the fish eggs into the mud and stir for 30 minutes to debond and then take it out. The mud is mixed with soil and water in a ratio of 1:8 and stirred evenly. After passing through a 100-mesh sieve, put it into the hatching tank and wash it with water. , the water velocity is 2.4m / s, and the water pressure is 0.07MPa;

[0025] S3. Hatching and stripping: add hatching medium, adjust pH value to 8, water temperature 34°C, hatch for 28h, then add hatching medium, adjust pH value to 8.5, water temperature 34°C, cultivate for 75h, the hatching medium is quality Butylated hydroxyanisole...

Embodiment 3

[0028] A method for removing egg membranes during grouper hatching: comprising the following steps:

[0029] S1. Hypotonic treatment: Add phosphate buffered saline solution to the hatching tank for rinsing. The volume ratio of phosphate buffered saline solution to egg cells is 32:18. After rinsing 3 times, soak the egg cells, mix and let stand for 10 minutes at 27°C;

[0030] S2. Stir and debond: Put the fish eggs into the mud and stir for 20 minutes to debond and then take it out. The mud is mixed with soil and water in a ratio of 1:6 and stirred evenly. After passing through a 90-mesh sieve, put it into the hatching tank and wash it with water. , the water velocity is 1.7m / s, and the water pressure is 0.05MPa;

[0031] S3, hatching and stripping: add hatching medium, adjust pH value to 6, water temperature 30°C, hatch for 24h, then add hatching medium, adjust pH value to 8, water temperature 30°C, cultivate for 65h, the hatching medium is quality Butylated hydroxyanisole, d...

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PUM

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Abstract

The invention provides a method for removing egg membranes in the hatching process of grouper, comprising the following steps: hypotonic treatment, stirring and debonding, hatching defilming, and egg membrane collection. The grouper of the present invention removes egg membranes in the hatching process The method scientifically adjusts the pH value, water temperature and the ratio of the culture agent, effectively treats the egg membrane, and achieves the maximum removal rate without affecting the water quality. The research on egg-membrane correlation of grouper fish has positive and important significance.

Description

technical field [0001] The invention relates to the field of biological membrane removal, in particular to a method for removing egg membranes during grouper hatching. Background technique [0002] Egg membrane is the general term for the acellular membrane that wraps animal egg cells. The morphological structure and composition of egg membranes in different animals are also quite different. Egg membrane plays an important role in the protection of egg cells and the process of sperm and egg recognition. As the largest group of invertebrates next to arthropods, molluscs have a variety of egg membrane structures. Overall, their egg membranes are thick and difficult to separate. [0003] Grouper roe and fish roe with egg membrane have a thick egg membrane, and the outermost end has a complex multi-layered protein shell with a certain thickness, and the egg membrane and the cytoplasmic membrane are closely attached, and it is difficult to separate and dissolve the egg membrane. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/17
CPCA01K61/17Y02A40/81
Inventor 黄春仁蔡惠明蔡春有蔡有森蔡建顺李伟蔡四川蔡金泉张国庆蔡智怀陈猛猛吴光灿陈艺斌刘赐福李伟峰罗磊蔡艺新王景宝
Owner HAINAN CHENHAI AQUATIC CO LTD