Unlock instant, AI-driven research and patent intelligence for your innovation.

Probe and method for identifying genetic sex of sipunculus nudus

A checkered worm and gender technology is applied in the field of identifying the genetic sex of checkered worms to achieve the effect of promoting improvement

Active Publication Date: 2020-01-31
HUAQIAO UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, there have been no reports on the female-specific molecular probes and their applications of P.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Probe and method for identifying genetic sex of sipunculus nudus
  • Probe and method for identifying genetic sex of sipunculus nudus
  • Probe and method for identifying genetic sex of sipunculus nudus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The technical solution of the present invention is further illustrated and described through specific embodiments below:

[0026] The instrument used in the present invention does not indicate the manufacturer, and all of them can be obtained through conventional commercial purchases. The invention includes three aspects: the screening of the female-specific gene fragment of the gridworm, the primer sequence of the female-specific gene segment of the gridworm and the identification of the genetic sex of the gridworm.

[0027] 1. Screening of female-specific gene fragments of P.

[0028] The female-specific gene fragment SEQ ID NO: 1 used in the embodiments of the present invention is derived from the sequencing results of the transcriptome of Acarina phalaenopsis completed by the Engineering Center of Molecular Medicine, Ministry of Education, Huaqiao University, and its sequence is:

[0029] GCAGATTCTTCGGGTACTGTTCGTGACGTCATGGCCAGAAAAAACCGTGAAGGCACCGTTGGCCAAAGTGTTGTCAC...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a probe and a method for identifying the genetic sex of sipunculus nudus. By means of a high-throughput sequencing means, a female specific gene segment with a sequence of SEQID NO:1 is identified by bioinformatic analysis, specific primers F1 and R1 for identifying the genetic sex of the sipunculus nudus are designed according to the gene fragment, and the primers only have amplification products in female individuals. The probe and the method are not limited by factors such as developmental stage and individual size of the sipunculus nudus and can rapidly, accuratelyand effectively identify the genetic sex of the sipunculus nudus by extracting a certain quantity of coelomic fluid; and bodies are not required to kill and can be applied too production and reproduction practice requirements after identification of the genetic sex. The probe and the method can effectively promote improvement of an artificial propagation technology for sipunculus nudus, and haveimportant theoretical significance and application values for breeding practice of the sipunculus nudus.

Description

technical field [0001] The invention relates to the genetic sex identification and sex control technology of the marine star worm in the field of aquatic biology technology and aquaculture, in particular to a method and a probe for identifying the genetic sex of the square star worm. Background technique [0002] Sipunculus nudus, commonly known as "sandworm", is widely distributed in the southeast coast of my country and is a rare and precious seafood treasure. This kind of organism is delicious and nutritious, and has important food and medicinal value. The gridworm used in production mainly comes from natural fishing. However, due to over-exploitation and environmental pollution, the resources of the starworm have declined sharply. Therefore, it is urgent to further explore the reproduction mechanism of the gridworm to improve the artificial breeding technology to meet the production requirements. need. [0003] The phalaenopsis is in vitro fertilization, dioecious, and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6879C12Q1/686C12N15/11
CPCC12Q1/6879C12Q1/686C12Q2600/124C12Q2565/125Y02A50/30
Inventor 李文华吴雅琴陈琪薇
Owner HUAQIAO UNIVERSITY