Method for identifying sex of white spotted pike

A technology for northern pike and identification methods, applied in biochemical equipment and methods, microbe measurement/inspection, DNA/RNA fragments, etc., can solve problems that have not been reported, and achieve the effect of saving manpower and material resources

Active Publication Date: 2022-04-12
XINJIANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chen Songlin (2008) from the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences found a sex-specific molecular marker for the star flounder, but the molecular biology techniques for sex-specific molecular markers and genetic sex identification of northern pike are currently unknown at home and abroad. See the report

Method used

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  • Method for identifying sex of white spotted pike
  • Method for identifying sex of white spotted pike
  • Method for identifying sex of white spotted pike

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The steps of PCR reaction test are as follows:

[0059] (a1) The PCR reaction uses the PCR reaction master mix (mix) of Tiangen Biochemical Technology Beijing Co., Ltd., and each reaction is 12.5 μL. The specific reaction system is shown in Table 1.

[0060]

[0061] (b1) The PCR reaction procedure is as follows: first, denaturation at 94°C for 10 minutes, and then 35 cycles of reaction, including denaturation at 94°C for 30 seconds, then annealing at 57°C for 30 seconds, extension at 72°C for 1 minute after annealing, and 72°C at the end of the reaction Extend for 10 min, and finally store at 4°C.

[0062] Agarose gel electrophoresis detection and gender identification:

[0063] (a2) Agarose gel electrophoresis:

[0064] Use an agarose horizontal electrophoresis instrument (Beijing Liuyi, DYCP-31DN) to carry out 2% agarose gel electrophoresis at a voltage of 150V to detect PCR amplification products, and use DL2000 as an electrophoresis maker (its bands are from t...

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Abstract

The invention discloses a sex identification method of pike vibrio molitor, and claims to protect a specific molecular marker of genetic sex of the pike vibrio molitor, the specific molecular marker has difference between X chromosome and Y chromosome of the pike vibrio molitor, and the difference can be used for identifying the genetic sex of the pike vibrio molitor. The invention overcomes the defects in the prior art, provides a specific molecular marker based on gender linkage, can simply and quickly realize genetic gender, and greatly saves manpower and material resources.

Description

technical field [0001] The invention belongs to fish genetic sex identification and sex control technology in the technical field of aquatic organisms, and is a molecular biological method for fish genetic sex identification that can be applied in fish parthenogenesis. Background technique [0002] my country's aquaculture industry has a long history, fish sex determination, differentiation mechanism and sex control technology breeding are of great significance in aquaculture research and application, many fish are sexually dimorphic, half smooth tongue sole (Cynoglossus semilaevis) Hippoglossus stenolepis) and other females grow significantly faster than males, and the realization of all-feminization breeding through sex control technology is one of the key technologies to improve its breeding production; Nile tilapia (Oreochromis mossambicus) is that males are significantly superior in growth. Females are expected to achieve full male production in breeding. Therefore, rese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6879C12Q1/6806C12N15/11
CPCY02A40/81
Inventor 张俊杰王顺哲杨倩汪泳昌刘璎慧刘祎
Owner XINJIANG AGRI UNIV
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