A kind of amniotic fluid cell culture medium

An amniotic fluid cell and culture medium technology, which is applied in the direction of cell culture active agent, culture process, animal cells, etc., can solve the problems of less cell mass, slow cell proliferation, and poor amniotic fluid culture effect.

Active Publication Date: 2021-03-19
河南赛诺特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amniotic fluid culture medium currently on the market has a poor effect on the culture of amniotic fluid cells, the cell proliferation is slow, and the cell clusters obtained after culture are less

Method used

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  • A kind of amniotic fluid cell culture medium
  • A kind of amniotic fluid cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The amniocyte culture medium in the present embodiment comprises the following components: insulin 2mg / L; glucagon 2mg / L; hydrocortisone 1nmol / L; sodium selenite 10nmol / L; colony-stimulating factor 2μg / L ; Estradiol 1nmol / L; Ferric nitrate 2mg / L; Ferric citrate 5mg / L; Fibroblast factor 0.5μg / L; Nerve growth factor 1μg / L; Epidermal growth factor 2μg / L; Human albumin 5mg / L; vitamin 2mg / L; human embryo trophoblast cell culture medium 50mL / L, and the balance is the basal medium F12 / DMEM.

[0027] The human embryo trophoblast cell culture medium is prepared by a method comprising the following steps: human embryo trophoblast cells are purchased from a professional primary cell isolation and culture company;

[0028] 1) The human embryo trophoblast cell count reaches 5×10 5 At 1 cell / mL, transfer to a T25 culture flask (expansion ratio at the same density) for culture, and culture in a carbon dioxide incubator at 37°C for 48 hours with complete culture medium.

[0029] 2) ...

Embodiment 2

[0033] The amniocyte culture medium in the present embodiment comprises the following components:

[0034] Insulin 1mg / L; Glucagon 5mg / L; Hydrocortisone 5nmol / L; Sodium Selenite 30nmol / L; Colony stimulating factor 10μg / L; Estradiol 5nmol / L; Ferric nitrate 1mg / L; Lemon Ferric acid 1mg / L; fibroblast factor 0.1μg / L; nerve growth factor 20μg / L; epidermal growth factor 20μg / L; human albumin 1mg / L; vitamin 5mg / L; human embryo trophoblast cell culture medium 70mL / L, and the balance is the basal medium F12 / DMEM.

[0035] The human embryo trophoblast cell culture medium used is the same as that in Example 1.

Embodiment 3

[0037] The amniocyte culture medium in the present embodiment comprises the following components:

[0038] Insulin 5mg / L; Glucagon 1mg / L; Hydrocortisone 3nmol / L; Sodium Selenite 20nmol / L; Colony stimulating factor 1μg / L; Estradiol 3nmol / L; Ferric nitrate 5mg / L; Lemon Ferric acid 3mg / L; Fibroblast factor 20μg / L; Nerve growth factor 0.1μg / L; Epidermal growth factor 0.1μg / L; Human albumin 3mg / L; Vitamin 1mg / L; Human embryo trophoblast cell culture 30mL / L, and the balance is the basal medium F12 / DMEM.

[0039] The human embryo trophoblast cell culture medium used is the same as that in Example 1.

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Abstract

The invention relates to an aminotic cell culture medium, and belongs to the technical field of cell culture media. The aminotic cell culture medium comprises the following components: a basal culturemedium, an aminotic cell culture additive and a human embryo trophoblast cell culture fluid, wherein the dosage of the human embryo trophoblast cell culture fluid is 30 to 70mL / L. The aminotic cell culture medium is added with the human embryo trophoblast cell culture fluid which is a natural composition obtained through cell culture, is a high-efficiency multi-factor mixture, and has an amnioticcell growth stimulation effect which is remarkably superior to a common culture medium component. The aminotic cell culture medium provided by the invention can effectively stimulate aminotic cell growth so as to obtain more cell clusters.

Description

technical field [0001] The invention relates to an amniotic fluid cell culture medium, which belongs to the technical field of cell culture medium. Background technique [0002] Amniocentesis is the most commonly used technique for invasive prenatal diagnosis. Doctors can obtain free cells from fetal skin, gastrointestinal tract, urinary tract, etc. by extracting amniotic fluid, and use these free cells to further analyze whether the fetal chromosome is abnormal. The operation is generally performed at 16 to 22 weeks, when the proportion of viable cells in the amniotic fluid is high. Extracting amniotic fluid is mainly to analyze the chromosomal composition of the fetus, the most important and common of which is Down syndrome. Some monogenic diseases, such as beta-thalassemia and hemophilia, can also be diagnosed by testing the genes (DNA composition) in the amniotic fluid cells. [0003] During amniocentesis, doctors can obtain free cells from fetal skin, gastrointestina...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/073
CPCC12N5/0605C12N2500/20C12N2500/24C12N2500/38C12N2501/11C12N2501/113C12N2501/115C12N2501/195C12N2501/22C12N2501/33C12N2501/335C12N2501/39C12N2501/998C12N2502/025
Inventor 翟晋豫柴素真张东辉张楠曹晓菲刘玲玲齐华
Owner 河南赛诺特生物技术有限公司
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