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A kind of Streptomyces venezuela and its identification method and application

A technology of Streptomyces venezuela and Streptomyces, which is applied in the field of microorganisms to achieve rapid and convenient identification

Active Publication Date: 2021-07-30
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the current problem is that in order to quickly and accurately obtain the characteristic information of Venezuelan zipper mold in the soil above the oil and gas fields, it is urgent to develop research on the acquisition of typical strains and the detection technology in the mixed system

Method used

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  • A kind of Streptomyces venezuela and its identification method and application
  • A kind of Streptomyces venezuela and its identification method and application
  • A kind of Streptomyces venezuela and its identification method and application

Examples

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Effect test

Embodiment 1

[0090] Example 1: Utilize Streptomyces venezuela SINOPEC22 to identify soil samples above Chunguang Oilfield.

[0091] (1) According to the 16S rDNA conserved sequence of Streptomyces venezuela SINOPEC22, four sets of 16S rDNA-specific primer pairs were designed. The specific sequences are:

[0092] Primer pair 1: F1:5'-GCTTAATTCGACGCAACGCGAAGAACCT-3',

[0093] R1: 5'-GCAATGCTGATCTGCGATTACTAGCAACTCC-3';

[0094] Primer pair 2: F2: 5'-CGGGTTGTAAACCTCTTTCAGCAGGAGGAA-3',

[0095] R2: 5'-ATCGCAGCTCTTTGTACCGGCCATTGTA-3';

[0096] Primer pair 3: F3: 5'-CGGGTTGTAAACCTCTTTCAGCAGGAGGAA-3',

[0097] R3: 5'-GCAATGCTGATCTGCGATTACTAGCAACTCC-3';

[0098] Primer pair 4: F4: 5'-GCAATCTGCCCTTCACTCTGGGACAAGC-3',

[0099] R4: 5'-TGATCTGCGATTACTAGCAACTCCGACTTCAT-3'.

[0100] The obtained target band sizes were: primer pair 1: 420bp, primer pair 2: 829bp; primer pair 3: 1036bp; primer pair 4: 1223bp.

[0101] (2) Extract the whole genome of microorganisms in the soil.

[0102] Weigh 0.5g o...

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Abstract

The invention belongs to the technical field of microbes, and in particular relates to Streptomyces venezuela and its identification method and application. The invention screens out a highly specific oil and gas indicator microorganism, Streptomyces venezuela, and quickly detects Streptomyces venezuela in the soil through specific primers, which solves the defects of short identification period and application range of common technologies, and is more rapid Convenient, by analyzing the abnormal concentration characteristics of microorganisms, it can predict oil and gas enrichment areas and oil and gas reservoirs. The present invention also provides a method for using the Streptomyces venezuelae to indicate the concentration of gaseous hydrocarbons floating in oil and gas reservoirs. The method uses improved primers to amplify the 16S rDNA conserved sequence of the Streptomyces venezuelae. The brightness of the strips can accurately and efficiently determine the concentration of gaseous hydrocarbons floating in the oil and gas reservoir.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a Streptomyces venezuela and its identification method and application. Background technique [0002] Microbial oil and gas exploration technology is a surface exploration technology derived from the principle of light hydrocarbon micro-leakage in oil and gas reservoirs. The light hydrocarbon part (C1-C5) in the oil and gas reservoir passes through the overlying sedimentary layer in the form of micro-leakage, and the gaseous hydrocarbon undergoes a long migration process, which makes the hydrocarbon content in the near-surface soil or water layer reach a higher level. concentration. This high concentration of gaseous hydrocarbons can also be utilized by microorganisms. The reproduction and growth of microorganisms specialized in utilizing light hydrocarbons are induced in the near-surface soil, and the types and concentrations of microorganisms in oil and gas areas are differe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/689C12Q1/64C12R1/61
CPCC12N1/20C12Q1/689C12N1/205C12R2001/61
Inventor 顾磊汤玉平许科伟杨帆高俊阳
Owner CHINA PETROLEUM & CHEM CORP
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