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Straw-degrading complex microbial inoculant containing candida parapsilosis and application of straw-degrading complex microbial inoculant

A Candida and compound bacterial agent technology, applied in the field of compound bacteria, can solve the problems of limited use, protein waste, and high operational requirements, and achieve the effect of high stability

Active Publication Date: 2020-02-25
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cottonseed cake is not only rich in resources and widely distributed, but also has high nutritional value. The protein content is about 40%, and the amino acid composition is also good. However, because cottonseed kernels and cottonseed cake contain toxic substances such as gossypol, its use is greatly restricted Over the years, about 80% of my country's cottonseed cake resources have been used as fertilizer and returned to the field, and less than 30% of the protein resources used as feed have caused a great waste of protein
However, most of the complex bacterial strains reported so far show lignocellulose degradation ability under high temperature conditions or require chemical pretreatment of degraded raw materials, which are to a certain extent
[0005] Higher operating requirements and higher energy consumption

Method used

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  • Straw-degrading complex microbial inoculant containing candida parapsilosis and application of straw-degrading complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The mutagenic screening of embodiment 1 verrucous Myromyces

[0021] (1) Morphological identification and screening

[0022] The soil in the forest area of ​​Changbai Mountain Nature Reserve was collected and cultivated on PDA medium by conventional methods. The mycelium of the strain was white flocculent at first, and grew divergently towards the periphery. After 8 days of culture, the color of conidia continued to deepen, and glue dots appeared; after 10 days of culture, the colony was in the shape of concentric rings, the conidia showed black glue spots, and the back of the colony appeared Hazel radial folds.

[0023] Use a puncher to get a 1cm-diameter bacterial block, inoculate it on an aniline blue selective medium, and culture it in an aerobic incubator at 30°C for 10 days, observe the fading of aniline blue, and select a strain with a fast fading rate and strong ability as candidate strains.

[0024] At the same time, in order to directional screen lignin-deg...

Embodiment 2

[0030] The low-temperature acclimatization of embodiment 2 bacterial strains

[0031] (1) Enrichment culture strains

[0032] Inoculate the strain into a Erlenmeyer flask filled with 150mL of PDA medium, add filter paper pieces with a length of 5cm and a width of 2cm, incubate at a constant temperature at 30°C for 1 hour and mix evenly, and make 10 -1 -10 -9 For dilution, 1 mL of each dilution was inoculated in PDA medium with filter paper as the only carbon source, and static enrichment culture was carried out at 28°C for 4 generations. Screen out the materials with short breakage time and high degree of ulceration of filter paper. PDA medium: potato 200g / L, glucose 20g / L, agar 15g / L, enrichment culture for 4 generations.

[0033] (2) Preliminary screening and domestication of low-temperature cellulose-degrading composite strains

[0034] Using the filter paper disintegration method, the above-mentioned enriched culture was inoculated into the filter paper strip medium to...

Embodiment 3

[0037] The preparation of embodiment 3 composite bacterial agents

[0038] Cellulase-producing Paenibacillus, xylanase-producing Alternaria, laccase-producing Mycobacterium verrucosus, lignin-peroxidase-producing Bacillus colloid, manganese-peroxidase-producing Lys Parapsilosis and Candida parapsilosis were activated in a conventional manner and cultured until the number of viable bacteria in the bacterial liquid reached 2.0×10 8 individual / mL.

[0039]Mix the bacterial solution according to the following mass ratio: 20% of Paenibacillus cellulase-producing bacteria, 20% of Alternaria xylanase-producing bacteria, 20% of Laccase-producing Mycobacterium verrucosus, lignin peroxidase glue 10% of Bacillus plasmogenus, 10% of manganese peroxidase-producing Layseia actinomyces, 20% of Candida parapsilosis, fully stirred and mixed to obtain a mixed bacterial liquid.

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Abstract

The invention provides a complex microbial inoculant containing candida parapsilosis and capable of effectively degrading straws at a low temperature. The complex microbial inoculant is composed of six strains, enzyme capable of efficiently expressing and decomposing cellulose, hemicellulose and lignin under the low-temperature condition is obtained through creative screening and accumulation fora long time, the characteristics of insufficient low-temperature enzyme activity, difficult straw decomposition and degradation and the like are broken through, the cotton straw decomposition days areincreased by 2-3 days in the environment with the outdoor temperature being 1-6 DEG C, and the complex microbial inoculant is particularly suitable for northern areas. When the composite bacterial system is cultured to 30 generations, the cotton straw degradation rates of the generations at 4 DEG C are not obviously different, and the stability is high.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a composite bacterium containing Candida parapsilosis that effectively degrades straw at low temperature and an application thereof. Background technique [0002] Cotton is one of the most important crops in the world, accounting for about 1 / 4 of the world's cotton output. The large output and low production cost make cotton products relatively cheap. The annual output of cottonseed cake in my country is about 4-5 million tons, accounting for 30% of the total output of various plant cakes. Cottonseed cake is not only rich in resources and widely distributed, but also has high nutritional value. The protein content is about 40%, and the amino acid composition is also good. However, because cottonseed kernels and cottonseed cake contain toxic substances such as gossypol, its use is greatly restricted Over the years, about 80% of my country's cottonseed cake resources have been used as...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N1/16C12R1/01C12R1/645C12R1/07C12R1/72
CPCC12N1/14C12N1/16C12N1/20
Inventor 孙旸陈光孙春玉林晓琼王刚苟泽昌
Owner JILIN AGRICULTURAL UNIV
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