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Bacillus amyloliquefaciens-containing composite microbial agent for low-temperature degradation of straw and application

A technology of Bacillus amylobacter and compound bacterial agent, which is applied in the field of microorganisms, can solve the problems of straw resources not being rationally developed and straws not easy to decompose, and achieve the effects of promoting potato growth, reducing the incidence of diseases and insect pests, and increasing production capacity

Active Publication Date: 2018-11-13
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on straw-degrading microorganisms is mainly focused on bacteria and fungi. Due to the long-term low temperature in the north, straw is not easy to decompose in a short period of time, resulting in the long-term lack of reasonable development of straw resources.

Method used

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  • Bacillus amyloliquefaciens-containing composite microbial agent for low-temperature degradation of straw and application
  • Bacillus amyloliquefaciens-containing composite microbial agent for low-temperature degradation of straw and application

Examples

Experimental program
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Effect test

Embodiment 1

[0020] The mutagenic screening of embodiment 1 verrucous Myromyces

[0021] (1) Morphological identification and screening

[0022] The soil in the forest area of ​​Changbai Mountain Nature Reserve was collected and cultivated on PDA medium by conventional methods. The mycelium of the strain was white flocculent at first, and grew divergently towards the periphery. After 8 days of culture, the color of conidia continued to deepen, and glue dots appeared; after 10 days of culture, the colony was in the shape of concentric rings, the conidia showed black glue spots, and the back of the colony appeared Hazel radial folds.

[0023] Use a puncher to get a 1cm-diameter bacterial block, inoculate it on an aniline blue selective medium, and culture it in an aerobic incubator at 30°C for 10 days, observe the fading of aniline blue, and select a strain with a fast fading rate and strong ability as candidate strains.

[0024] At the same time, in order to directional screen lignin-deg...

Embodiment 2

[0030] The low-temperature acclimatization of embodiment 2 bacterial strains

[0031] (1) Enrichment culture strains

[0032] Inoculate the strain into a Erlenmeyer flask filled with 150mL of PDA medium, add filter paper pieces with a length of 5cm and a width of 2cm, incubate at a constant temperature at 30°C for 1 hour and mix evenly, and make 10 -1 -10 -9 For dilution, 1 mL of each dilution was inoculated in PDA medium with filter paper as the only carbon source, and static enrichment culture was carried out at 28°C for 4 generations. Screen out the materials with short breakage time and high degree of ulceration of filter paper. PDA medium: potato 200g / L, glucose 20g / L, agar 15g / L

[0033] Natural corn stalk powder shaker culture: Weigh 5 g of the strain source sample and add it to 100 mL corn stalk powder medium with natural corn stalk powder as the only carbon source (replacing the filter paper in the PDA medium with corn stalk powder, culture at 28°C and 150 rpm On ...

Embodiment 3

[0045] The preparation of embodiment 3 composite bacterial agents

[0046] Cellulase-producing Paenibacillus, xylanase-producing Alternaria, laccase-producing Mycobacterium verrucosus, lignin-peroxidase-producing Bacillus colloid, manganese-peroxidase-producing Lys Bacillus amyloliquefaciens and Bacillus amyloliquefaciens were activated in a conventional manner and cultured until the number of viable bacteria in the bacterial solution reached 2.0×10 8 pieces / ml.

[0047] Mix the bacterial solution according to the following mass ratio: 20% of Paenibacillus cellulase-producing bacteria, 10% of Alternaria xylanase-producing bacteria, 20% of Laccase-producing Mycobacterium verrucosus, lignin peroxidase glue 10% of Bacillus plasmoides, 10% of Manganese peroxidase-producing Layseia actinomycetes, 30% of Bacillus amyloliquefaciens, fully stirred and mixed to obtain a mixed bacterial liquid.

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Abstract

The invention provides a Bacillus amyloliquefaciens-containing composite microbial agent for low-temperature degradation of straw. The composite agent comprises six strains, maturity period of maize straw is 2-3 days earlier in an outdoor environment at 1-6 DEG C, and the agent is particularly suitable for the northern area. The composite agent has higher stimulant metabolite production capacity and can secrete plant growth hormones and promote plant growth; the composite agent can secrete phytase and organic acid for degrading phytic acid and phosphorus mineral in soil; the composite agent can produce various metabolites related to bacteriostasis, and has a strong inhibition effect on fungi and bacteria. Compound microorganisms play a synergistic role in soil and complement one another, so that soil structure is improved effectively, soil fertility is improved, and yield is increased.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a low-temperature degrading straw microbial composite bacterial agent containing Bacillus amyloliquefaciens and its application. Background technique [0002] Corn stalks are grain, economy and feed crops mainly used as feed, and corn stalks are also important production resources for industrial and agricultural production. As a species, corn stover is rich in nutrients and available chemical components, and can be used as a raw material for livestock feed. A large number of studies have shown that returning straw to the field can effectively increase the content of soil organic matter, improve soil, and improve soil fertility. It is of great significance for alleviating the imbalance of nitrogen, phosphorus, and potassium fertilizer ratios in China. However, due to China's large population and limited arable land, the multi-cropping index of arable land is high, the stubble time i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F15/00C05F17/00C12R1/07C12R1/645C12R1/01
CPCC05F11/00C05F17/00C12N1/14C12N1/20C05F11/08Y02W30/40
Inventor 孙旸陈光卢芳林晓琼戴祁霏苟泽昌孙春玉于潇潇王聪
Owner JILIN AGRICULTURAL UNIV
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