Quantitative analysis method for twelve components of Huang Qi Jian Zhong pills in rat plasma

One kind of quantitative analysis, twelve kinds of technology, applied in the field of the detection of traditional Chinese medicine components in biological samples, can solve the problem of unreported quantitative detection, and achieve the effects of accurate content, good separation, and short analysis time

Inactive Publication Date: 2020-02-28
SHANXI UNIV
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Problems solved by technology

At present, there are no reports on the methods of in vivo quantitative dete...

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  • Quantitative analysis method for twelve components of Huang Qi Jian Zhong pills in rat plasma
  • Quantitative analysis method for twelve components of Huang Qi Jian Zhong pills in rat plasma
  • Quantitative analysis method for twelve components of Huang Qi Jian Zhong pills in rat plasma

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Embodiment 1

[0033] In this embodiment, a method for quantitative analysis of twelve components of Huangqi Jianzhong Pill in rat plasma comprises the following steps:

[0034] (1) Rat plasma samples were processed: Huangqi Jianzhong Pills were orally administered to rats, blood was collected from the orbit 2 hours later, and plasma samples were collected. Take 200 μL of plasma sample, add 50 μL of internal standard working solution, then add 800 μL of acetonitrile, vortex for 5 minutes, centrifuge at 4°C, 13000 rpm for 15 minutes, take 1000 μL of supernatant in another 1.5mL centrifuge tube, place in high-speed centrifuge to concentrate In the instrument, keep warm at 40°C, blow dry, redissolve in 100 μL of methanol, vortex for 1 minute, centrifuge at 4°C, 13000 rpm for 10 minutes, take the supernatant to obtain the required sample solution.

[0035] (2) Preparation of reference substance stock solution: take liquiritin, isoliquiritin, liquiritigenin, actecoside, formononetin, pteroside, a...

Embodiment 2

[0047] Investigation on the determination method of twelve components in Huangqi Jianzhong Pills in rat plasma:

[0048] The sample solution of following experiment all adopts following method to prepare:

[0049] 1) Establishment of standard curve and linear range test

[0050] Dilute the mixed standard working solution to a serial concentration of 2 times, 5 times, 10 times, 20 times, 50 times, 100 times and 200 times the final concentration. Using the proposed method, Waters ACQUITY UPLC HSS T3 (2.1mm×100mm, 1.8μm) chromatographic column was used to detect with Agilent1290 ultra-high performance liquid chromatography coupled with Q3200 tandem mass spectrometry to draw a standard curve and obtain the correlation coefficient and linear range of each component , limit of quantification and limit of detection results are shown in Table 2.

[0051] Table 2 Standard curve, correlation coefficient, linear range, quantification limit and detection limit results of twelve kinds of...

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Abstract

The invention relates to a quantitative analysis method for twelve components of Huang Qi Jian Zhong pills in rat plasma, and belongs to the field of biological analysis. A rat plasma sample is treated by protein precipitation, and the contents of twelve components of Huang Qi Jian Zhong pills in rat plasma is detected in a UPLC-MS/MS (ultra-high performance liquid chromatography-mass spectrometry) method by taking kaempferol as the internal standard. The detection method of the invention is high in sensitivity, specificity and stability, the recovery rate reaches 108.74%, the requirement forthe recovery rate can be met, and the detection efficiency can be improved. Besides, the detection method is high in reproducibility and short in analysis time, can meet the requirement for detectingthe concentration of the Huang Qi Jian Zhong pills in the rat plasma, and can be used for pharmacokinetic research and bioequivalence research of the Huang Qi Jian Zhong pills.

Description

technical field [0001] The invention relates to the technical field of detection of components of traditional Chinese medicines in biological samples, in particular to a quantitative analysis method for twelve components of Huangqi Jianzhong Pills in rat plasma. Background technique [0002] Huangqi Jianzhong Pill is derived from Zhongjing Jingfang Huangqi Jianzhong Decoction. The whole prescription is composed of five herbs of Astragalus, cinnamon, white peony root, roasted licorice and jujube. Clinically, it is mainly used to invigorate qi and dispel cold, invigorate the stomach and harmonize the middle. Modern pharmacological studies have shown that it is effective in treating gastrointestinal diseases caused by spleen deficiency; in addition, studies have shown that Huangqi Jianzhong Pills also have sedative and antispasmodic effects, lowering blood sugar, regulating blood components, and regulating immune function. At present, there are no reports on the methods of in ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/045
Inventor 刘月涛贾璐秦雪梅
Owner SHANXI UNIV
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