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The fungus stagonospora neglecta HT01 and its applications

A technology of fungi and inoculants, applied in fungi, biological water/sewage treatment, microorganisms, etc., can solve the problems of intolerance to strong alkali conditions, high cultivation and action temperature, etc.

Active Publication Date: 2022-07-22
HUATIAN ENG & TECH CORP MCC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the microbial strains disclosed in the above patent inventions can only deal with high concentrations of Cr(VI) pollution, and require higher cultivation and action temperatures (about 30°C), and are not tolerant to strong alkali conditions

Method used

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  • The fungus stagonospora neglecta HT01 and its applications
  • The fungus stagonospora neglecta HT01 and its applications
  • The fungus stagonospora neglecta HT01 and its applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Experiment on the treatment of low-concentration chromium-containing wastewater (1-10mg / L) by Stagonospora neglecta HT01 fungi

[0037] Different liquid Chapkin's medium with gradient Cr(VI) was prepared (sodium nitrate 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sucrose 30g, distilled water 1000mL, dichromate The amount of potassium acetate added was such that the Cr(VI) concentrations of the three gradients were 1, 5, and 10 mg / L, respectively), which were divided into three conical flasks and inoculated with the same amount of Stagonosporaneglecta HT01 bacteria. After shaking on the shaker for 24 hours, take samples to measure its Cr(VI) concentration, compare the difference before and after, and calculate the reduction rate. The results are as follows: figure 2 shown.

[0038]

[0039] The results show that Stagonospora neglecta HT01 has a good reduction effect on low concentration (1-10mg / L) Cr...

Embodiment 2

[0041] Treatment experiment of low-concentration chromium-containing wastewater (10 mg / L) by Stagonospora neglecta HT01 fungus in strong alkaline environment (pH=13)

[0042] Liquid Chapkin's medium containing Cr(VI) under strong alkaline conditions (sodium nitrate 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sucrose 30g, distilled water 1000mL, The amount of potassium dichromate added was such that the concentration of Cr(VI) was 10 mg / L, and the pH was adjusted to 13 with sodium hydroxide). The shaker was shaken and sampled every 2 days to measure its Cr(VI) concentration. The results are as follows image 3 shown.

[0043] The results show that after 14 days of continuous testing, Stagonospora neglecta HT01 can reduce 10mg / L of Cr(VI) to less than 0.5mg / L, meeting the requirements of four types of surface water. The reduction rate was slow in the first few days and gradually increased.

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Abstract

The invention discloses a fungus Stagonospora neglecta HT01 and its application. It is the fungus Stagonospora neglecta HT01, which is preserved in the General Microbiology Center of China Microorganism Culture Collection and Management Committee, and its address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The number of the deposit register is CGMCC No. 17596, and the deposit date is April 15, 2019. The fungus HT01 provided by the invention can tolerate a strong alkaline environment (pH is less than or equal to 13), and has a high reduction efficiency for low-concentration hexavalent chromium, which is reduced from 10 mg / L to below 0.5 mg / L within 24 hours. The fungus Stagonospora neglecta HT01 provided by the invention has a reducing effect on hexavalent chromium, and can be used for repairing water bodies polluted by hexavalent chromium.

Description

technical field [0001] The invention relates to the technical field of environmental microbial remediation, in particular to a fungus Stagonosporaneglecta HT01 and its application. Background technique [0002] With the development of economy and industry, a large amount of heavy metals, such as chromium and other substances, enter the natural environment and cause environmental pollution, which has attracted widespread attention. Hexavalent chromium is easily absorbed by the human body and accumulates continuously. It can enter into cells and cause teratogenic, carcinogenic and other pathological changes, which is more harmful to humans. [0003] The treatment of hexavalent chromium pollution usually adopts chemical methods to convert it into trivalent chromium state with minimal toxicity, or adopts physical methods for recycling. However, these methods will bring disadvantages such as subsequent processing trouble, high cost, and secondary pollution. In recent years, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C02F3/34C12R1/645C02F101/22
CPCC02F3/347C02F2101/22C12R2001/645C12N1/145
Inventor 孟溪骆守鹏崔倩倩陶文达高卫民张志杨
Owner HUATIAN ENG & TECH CORP MCC