Anti-amantadine amd single-chain antibody scfv and its preparation method and application

A single-chain antibody and amantadine technology, which is applied in the field of amantadine AMD single-chain antibody scFv and its preparation, can solve the problems of unreported and unreported patents
CN110872355BActive Publication Date: 2021-08-24CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2021-08-24

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Abstract

The invention discloses an anti-amantadine AMD single-chain antibody and its preparation and application. The present invention can obtain the heavy chain and light chain variable region genes of the antibody from the hybridoma cell line 3F2 secreting the monoclonal antibody of amantadine AMD through genetic engineering, and construct the prokaryotic expression plasmid by recombining the two genes The carrier is transformed into Escherichia coli to express the scFv antibody active fragment that can specifically recognize AMD. It is proved by experiments that the recombinant single-chain antibody scFv prepared by the present invention has specific recognition and binding activity with amantadine AMD. The amantadine residue detection can be realized by using the recognition and binding activity of the recombinant single-chain antibody of the present invention to amantadine, and the constructed transformation vector plasmid is easy to preserve and mass-produce.
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Description

technical field

[0001] The present invention relates to an amantadine AMD single-chain antibody scFv and a preparation method thereof, in particular to the protein sequence of the single-chain antibody and its coding gene sequence, a vector containing the coding gene and a method for preparing the single-chain antibody , belonging to the field of biological products. Background technique

[0002] Amantadine and other antiviral drugs are used in the prevention and treatment of influenza A virus in animal husbandry, but the abuse of amantadine is likely to cause drug resistance, promote strain mutation and endanger human health, and has a series of neurotoxicity, etc. Side effects, such as causing nervousness, anxiety, nightmares and other symptoms. Announcement No. 560 of the Ministry of Agriculture of my country in 2005 clearly stipulates that antiviral drugs such as amantadine and rimantadine are prohibited from being used in veterinary medicine. Livestock and poultry. Th...

Claims

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