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Intermittent flow-type electrotransfection device

An electrotransfection, intermittent technology, applied in the field of electrotransfection, can solve the problems of affecting the uniformity of the electric field and the instability of the electrotransfection effect, and achieve novel structural design, reduce the fluctuation of electrotransfection efficiency, and suppress the generation of air bubbles Effect

Pending Publication Date: 2020-03-17
ETTA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, the flow electrotransfection device disclosed above will generate a large number of air bubbles during the actual electrotransfection process, and the generated air bubbles will adhere to the electrode plate and affect the uniformity of the electric field, resulting in unstable electrotransfection effect.
After searching, the inventor found that in the field of flow electrotransfection, the technical problem of bubbles generated during electrotransfection has not been solved

Method used

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  • Intermittent flow-type electrotransfection device
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Overall structure design (1)

[0036] The intermittent flow electrotransfection device provided by the present invention is as figure 1 As shown, the nozzle at one end of the pipe body (3) is set as a pipe body sealing end (9), and the first electrode (1) and the second electrode (2) are respectively arranged in parallel on the inner wall of the pipe body (3), The first piston (7) is arranged at the other end of the pipe body (3), the first piston (7) is fixedly connected with the pressurizing device (6), and the pressurizing device (6) can pressurize The first piston (7) moves from the non-working position to the working position, and the pressurizing device is preferably a hydraulic rod, so that the liquid carried inside the cavity bears a certain pressure. The pipe body (3) is provided with a vent pipe (12), and a vent valve (13) is provided at the joint between the vent pipe (12) and the pipe body (3). The electric pulse is applied to the first electrode (1) and the...

Embodiment 2

[0038] Overall Structural Design (2)

[0039] The intermittent flow electrotransfection device provided by the present invention is as figure 2 As shown, the first piston (7) and the second piston (8) are arranged at the two ends of the tube body (3), and the first electrode (1) and the second electrode (2) are arranged in parallel on the tube body The inner wall of the first piston (7) and the second piston (8) set the working position and the non-working position, and the first piston and the second piston are fixedly connected with the pressurizing device (6) respectively, and the pressurizing device ( 6) A hydraulic rod is preferred, and the pressurizing device (6) can move the first piston (7) and the second piston (8) from the non-working position to the working position respectively, so that the liquid inside the cavity can bear a certain pressure. The electric pulse is applied to the first electrode (1) and the second electrode (2), and the electric pulse forms an el...

Embodiment 3

[0041] Liquid intermittent feeding method and pressurization method

[0042] When feeding liquid, open the sample inlet check valve (10), close the sample outlet check valve (11), open the vent valve (13), and use the air pump to pump out the gas with the same volume as the tube body (3) through the vent tube (12). , the target liquid sample enters the cavity from the liquid inlet pipe (4); or the gas of the same volume as the pipe body (3) is extracted through the vent pipe (12) without using the air pump, and the target liquid sample is drawn from the liquid inlet pipe ( 4) Press into the cavity; after the cavity is full, close the sampling check valve (10) and the ventilation valve (13). Apply specific pressure to the first piston (7) and / or the second piston (8) through the pressurizing device (6) to move it from the non-working position to the working position to complete the pressurization of the liquid and the electric rotation of the liquid Dyeing is carried out under...

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Abstract

The invention discloses an intermittent flow-type electrotransfection device, which comprises a tube body, a first electrode, a second electrode, and a first piston, and characterized in that the first electrode and the second electrode are arranged on the inner wall of the tube body in parallel; wherein one end of the tube body is sealed; the first piston is positioned at the opening of the tubebody; wherein a cavity for accommodating a liquid sample is formed in the first piston and the tube body, the tube body is provided with at least one liquid inlet / outlet and at least one liquid inlet / outlet valve, the liquid inlet / outlet valve is positioned at the liquid inlet / outlet of the tube body to control the opening and closing of the liquid inlet / outlet, and the first piston can relativelymove along the tube body. According to the flow-type electrotransfection device, the stability of the electrotransfection device can be improved, and the electrotransfection efficiency is improved.

Description

technical field [0001] The invention relates to the field of electrotransfection, and relates to an intermittent flow electrotransfection device. More specifically, the invention relates to an intermittent flow electrotransfection device that suppresses the generation of air bubbles in electrotransfection by means of piston movement and pressurization. dyeing device. Background technique [0002] The cell membrane is a layer of thin film surrounding the periphery of the cell, which is a permeability barrier for the selective material exchange between the cell and the outside world. The cell membrane makes the cell an independent living unit and has a relatively stable internal environment. Some substances in the surrounding environment can pass through the cell membrane, while others cannot. Cells can absorb nutrients from the surrounding environment through the cell membrane, discharge metabolites, and make the transport of substances reach a state of balance. Therefore,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42
CPCC12M35/02
Inventor 戴晓兵马红杨昌陈
Owner ETTA BIOTECH