A Lentinus edodes strain Nongxiang No. 5 suitable for industrial cultivation and its molecular identification method

An identification method, the technology of shiitake mushrooms, applied in the field of shiitake mushrooms, can solve the problems of difficulty in guaranteeing yield and quality, instability, etc., and achieve good development and application prospects, intuitive judgment, and good flower shape effects

Active Publication Date: 2021-04-02
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

With the continuous upgrading of the shiitake mushroom industry and the change of the environment and climate in recent years, the traditional cultivation mode of shiitake mushrooms has been exposed to instability, "depending on the sky", and the difficulty in ensuring output and quality has become more and more serious. It has become more difficult to adapt to the increasingly fierce shiitake mushroom industry. Competitive requirements, factory production characterized by mechanized production, intelligent control, and annual cultivation is the direction for the upgrading and development of the shiitake mushroom industry

Method used

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  • A Lentinus edodes strain Nongxiang No. 5 suitable for industrial cultivation and its molecular identification method
  • A Lentinus edodes strain Nongxiang No. 5 suitable for industrial cultivation and its molecular identification method
  • A Lentinus edodes strain Nongxiang No. 5 suitable for industrial cultivation and its molecular identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the breeding of shiitake mushroom 'Nongxiang No. 5'

[0027] The breeding of shiitake mushroom 'Nongxiang No. 5' comprises the following steps:

[0028] (1) Conduct fruiting experiments on mushrooms 'L26', 'L087', 'L.0344', and 'L0227', collect their spores, and conduct spore germination experiments to obtain 4 single-spore strains of mushroom strains. Each strain Select 2 single-spore strains with good growth each, totaling 8 single-spore strains.

[0029] (2) Select mushroom strains 'Nongxiang 2', '808', 'Minfeng 1', 'Cr33', 'Shenxiang 10', 'L18', 'L236', 'Wuxiang 1', 10 'L0249' and 'L.0359' were paired with 8 single strains respectively, and the number of paired combinations was 80, and 63 hybrids were obtained.

[0030] (3) After the 63 hybrids were continuously transferred on PDA medium for 3 times, the mycelial growth rate was observed, 23 hybrids with slower growth speed were eliminated, and the remaining 40 hybrids were used to make strains for m...

Embodiment 2

[0039] The factory cultivation of shiitake mushroom 'Nongxiang No. 5' (CCTCC NO: M 20191018) includes the following steps:

[0040] (1) Production of shiitake mushroom 'Nongxiang No. 5' strain

[0041] The 'Nongxiang No. 5' strain was activated twice on PDA culture, then transferred to the regular medium of the original seed of Lentinus edodes, and cultivated at a constant temperature of 25°C until the original seed bottle was overgrown. After the original seed grows well, the liquid strain is produced according to the method of Ma Qiufeng (Ma Qiufeng. Production test and advantage analysis of the liquid strain of Lentinus edodes "18". Fujian Hot Work Technology, 2017, 42 (2): 21-22), and the obtained The liquid strain of 'Nongxiang No. 5'.

[0042] (2) Bacteria package production, culture, color change and fruiting management of shiitake mushroom 'Nongxiang No. 5'

[0043] The material selection, various raw material formulas, bagging, disinfection and sterilization of the ...

Embodiment 3

[0050] Example 3: Molecular marker identification method of Lentinus edodes 'Nongxiang No. 5' (CCTCC NO: M 20191018)

[0051] The molecular marker identification method of shiitake mushroom 'Nongxiang No. 5' comprises the following steps:

[0052] (1) Mycelia culture and collection

[0053] 1. Transfer the mushrooms 'Nongxiang No. 5', '808' and 'L26' to a 250 ml wide-mouthed triangular flask containing 100ml of PDA liquid medium, wherein the mushrooms '808' and 'L26' were purchased in the market;

[0054] 2. Place on a shaker at 25°C with a rotation speed of 90-130 r / min and incubate for 5-8 days;

[0055] 3. Filter the cultivated mycelia with gauze, rinse the mycelium with distilled water, and absorb the water with filter paper;

[0056] 4. Weigh 0.5-1.3 g of mycelium, wrap it with filter paper, and store it at -20°C for later use.

[0057] (2) Genomic DNA extraction by CTAB method

[0058] The specific operation steps for the extraction of genomic DNA from mushrooms 'Non...

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Abstract

The invention provides a shiitake mushroom 'Nongxiang No. 5' and a molecular marker identification method thereof, belonging to the field of shiitake mushrooms. The present invention uses shiitake mushroom '808' and shiitake mushroom 'L26' as parents to hybridize and breed strains with good stability, high fruiting temperature, short fruiting time, good fruiting body shape, short inter-tide times, and high yield. 'Nongxiang No. 5' shiitake mushroom strain with good development and application prospects. The strain was deposited in the China Center for Type Culture Collection on December 5, 2019, with the preservation number CCTCC NO:M 20191018. At the same time, a molecular marker identification method with strong specificity and easy operation is provided.

Description

technical field [0001] The invention belongs to the field of shiitake mushrooms, and in particular relates to a shiitake mushroom 'Nongxiang No. 5' and a molecular marker identification method thereof. Background technique [0002] mushroom Lentinus edodes Also known as flower mushroom, winter mushroom, incense letter, mushroom, etc., it has high nutritional value and delicious taste. It is a kind of edible fungus widely known by people, and it is also the edible fungus with the largest output in my country. With the continuous upgrading of the shiitake mushroom industry and the change of the environment and climate in recent years, the traditional cultivation mode of shiitake mushrooms has been exposed to instability, "depending on the sky", and the difficulty in ensuring output and quality has become more and more serious. It has become more difficult to adapt to the increasingly fierce shiitake mushroom industry. In order to meet the requirements of competition, factory ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01H15/00C12Q1/6895C12Q1/6858C12N15/11C12R1/645
CPCA01H15/00C12Q1/6858C12Q1/6895C12Q2600/156C12N1/145C12R2001/645C12Q2531/113C12Q2565/125
Inventor 刘新锐吴小平谢宝贵邓优锦
Owner FUJIAN AGRI & FORESTRY UNIV
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