Method for identifying base source of trichosanthes kirilowii maxim formula granules

A technology of formula granules and Gualou formula, which is applied in the field of source identification of Gualou formula granules, can solve the problem of being unable to distinguish between Trichosanthes formula granules and double-sided Trichosanthes formula granules, and achieve the effect of simple operation and strong specificity

Pending Publication Date: 2020-03-20
BEIJING KANGRENTANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defect that the prior art cannot distinguish the Trichosanthes formula granules and the double-sided Trichosanthes formula granules, thereby providing a method for identifying the source of the Trichosanthes formula granules

Method used

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  • Method for identifying base source of trichosanthes kirilowii maxim formula granules
  • Method for identifying base source of trichosanthes kirilowii maxim formula granules
  • Method for identifying base source of trichosanthes kirilowii maxim formula granules

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Experimental program
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Effect test

Embodiment 1

[0034] This embodiment provides a method for identifying the source of Trichosanthes formula granules, including the following steps:

[0035] (1) Determine the liquid chromatography conditions:

[0036] Chromatographic column: Octadecylsilane bonded silica gel is used as filler, the filler particle size is 1.7μm, column length is 100mm, inner diameter is 2.1mm, column temperature: 35°C;

[0037] Mobile phase: mobile phase A is methanol, mobile phase B is 0.1% formic acid aqueous solution, the flow rate is 0.35mL / min, gradient elution is adopted, and the gradient elution procedure is shown in Table 1 below:

[0038] Table 1 Mobile phase gradient elution program

[0039]

[0040] Injection volume: 1 μL; TUV Detector ultraviolet detector.

[0041] (2) Preparation of reference solution and test solution

[0042] Take 1 g of Trichomonas reference medicinal material, add 10 mL of water, ultrasonically extract for 30 minutes, allow to cool to room temperature, shake well, filt...

experiment example 1

[0068] Experimental Example 1 Precision Inspection Test

[0069] Get Trichosanthes formula granules (batch number: DF180209-062550-18), make the test solution according to the method of Example 1 step (2), continuously inject 6 times, each 1 μ L, according to Example 1 step (1) The chromatographic conditions are detected, the chromatogram is recorded, the common characteristic peaks are found, and the characteristic spectrum is obtained. Taking peak No. 5 as a reference peak, calculate its relative peak area and relative retention time, and calculate the RSD value. The results are shown in Tables 5 and 6 below.

[0070] Table 5 retention time and relative retention time table

[0071]

[0072] Table 6 Peak area and relative peak area table

[0073]

[0074] From the data in the above table, it can be seen that the relative retention time RSD of each characteristic peak is in the range of 0.03% to 1.25%, and the relative peak area RSD is in the range of 0.65% to 2.39%,...

experiment example 2

[0075] Experimental example 2 Stability investigation test

[0076] Take Trichosanthes formula granules (batch number: DF180209-062550-18), prepare the test solution according to the method of step (2) of Example 1, draw 1 μL at 0, 2, 4, 6, 8, 10, and 12 hours respectively, Inject and detect by the detection condition of embodiment 1 step (1), record chromatogram, take No. 5 peak as reference peak, calculate its relative peak area and relative retention time, and calculate RSD. The results are shown in Tables 7 and 8 below.

[0077] Table 7 retention time and relative retention schedule

[0078]

[0079] Table 8 Peak area and relative peak area table

[0080]

[0081] As can be seen from the data in the above table, the relative retention time RSD of each characteristic peak is in the range of 0.00% to 0.76%, and the relative peak area ratio RSD is in the range of 1.02% to 2.06%, indicating that the chemical composition of the test solution is within 12 hours. Good st...

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Abstract

The invention relates to the technical field of traditional Chinese medicine formula granule detection. The invention discloses a method for identifying a base source of trichosanthes kirilowii maximformula granules. According to the method, a methanol-0. 1% formic acid aqueous solution is used as a mobile phase; gradient elution is combined with liquid chromatogram analysis detected by an ultraviolet fluorescence detector to obtain a specific chromatogram of the trichosanthes kirilowii maxim formula granules. Taking a vanillic acid chromatographic peak of a No.5 chromatographic peak as a reference peak; according to the method, the base source of the trichosanthes kirilowii maxim formula granules can be judged according to the ratio of the chromatographic peak 3 to the chromatographic peak 4, the operation is simple, convenient and efficient, the vacancy in the technical field of recognition of the base source of the trichosanthes kirilowii maxim formula granules is made up, and themethod can be used as a special recognition method for the trichosanthes kirilowii maxim formula granules or bilateral trichosanthes kirilowii maxim formula granules.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicine formula granules, in particular to a method for identifying the source of Gualou formula granules. Background technique [0002] Trichosanthes kirilowii Maxim. or Trichosanthes rosthornii Harms is the dry mature fruit of Cucurbitaceae Trichosanthes kirilowii Maxim. Its peel, seeds and roots are used as medicine with Trichosanthes kirilowii Maxim. According to textual research, Gualou was first recorded in "Shen Nong's Materia Medica", listed as a Chinese medicine, and is a commonly used drug for clearing away heat and detoxifying in traditional Chinese medicine. The original plant of Trichosanthes used in past dynasties is a vine, with tendrils, single leaves, 3 to 5 lobes or no lobes, round or oblong fruit, stylopodium or no apex, yellow fruit after ripening, and the morphological description conforms to Cucurbitaceae plants Combining with the distribution area o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/54G01N30/74G01N30/88
CPCG01N30/02G01N30/06G01N30/14G01N30/54G01N30/74G01N30/88G01N2030/027G01N2030/062G01N2030/8809
Inventor 付静张志强高扬姜筱璇
Owner BEIJING KANGRENTANG PHARMA
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