GABA and electrophysiological micro-nano synchronous sensing detection chip and preparation method thereof

Abstract

The disclosure provides a GABA and electrophysiological micro-nano synchronous sensing detection chip and a preparation method thereof, wherein the micro-nano synchronous sensing detection chip includes: a base layer, a conductive layer and an insulating layer; the base layer is a flexible material, and the base layer Overlaid on the bottom support; the conductive layer is formed on the base layer, and is used to synchronously detect the changes of the inhibitory neurotransmitter GABA and nerve electrical signals in many different sub-regions of the hippocampal structure of the rat; the conductive layer includes: detection sites group, the distribution position matches the size of the rat hippocampus, and the detection site group radiates along the diagonal direction of the detection area and is distributed in different sub-regions of the rat hippocampus; the insulating layer is a flexible material, and the insulating layer is formed on the On the basal layer outside the region where the group of detection sites is located. The disclosure can simultaneously detect inhibitory neurotransmitters and electrical signals, combined with micro-processing technology, nano-materials and bio-sensitive thin film technology, to help discover and understand neural information networks and the pathogenic mechanism of biblical degenerative diseases.

Description

technical field [0001] The disclosure relates to the field of micro-processing technology of biosensors, in particular to a micro-nano synchronous sensing and detection chip for epileptic hippocampal slices of γ-aminobutyric acid and neural electrical activity and a preparation method thereof. Background technique [0002] The brain is composed of a large number of synapses, and the synapses carry out the interactive transduction of nerve signals through the two transmission methods of neurotransmitters and nerve electrical signals to complete our daily behavior, motor learning and other functional activities. Abnormal release of neurotransmitters between synapses or abnormal discharge of electrical signals may indicate the existence of potential neurological diseases. Simultaneously detecting the changes of the two dual-mode signals will help to obtain neurocytopathy in advance, and help to grasp and understand the pathogenic mechanism of neurological diseases and find more...

AI Technical Summary

Problems solved by technology

The detection of GABA neurotransmitter has caused great difficulties in real-time detection in vivo due to its lack of electroactive groups and unique specific enzymes

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