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A kind of detection method of capsaicin

A capsaicin and fluorescence detection technology, applied in the detection field, can solve the problems of expensive enzyme-linked immunoassay detection kits, easy environmental interference, metal ion interference, etc.

Active Publication Date: 2022-02-08
杭州柘大飞秒检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the electrochemical method must add various electrolytes as conductive substances, and the metal ions in the oil may interfere
However, ELISA kits are expensive, and the activity of enzymes is easily disturbed by the environment.

Method used

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  • A kind of detection method of capsaicin
  • A kind of detection method of capsaicin
  • A kind of detection method of capsaicin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of the benzohydrazone aluminum complex in this example is 0.3843 g (1 mmol) of N-((7-hydroxy-2-oxo-2H-benzopyran-3-one) base-benzylidene) benzene Hydrazone (purchased reagent) and 0.0443g (0.33mmol) of anhydrous aluminum chloride were mixed in 100mL of absolute ethanol and refluxed for 120 minutes, cooled to 50°C, 1 / 2 of the solvent was removed by rotary evaporation, and cooled to obtain Pale yellow crystals were washed with ethanol several times and dried in vacuo to obtain 0.28 g of the product with a yield of 66%. from figure 1 The infrared spectrum can be found, 3427cm -1 The coupling broad peak of hydroxyl and amino is at 1597 cm -1 For the carbonyl peak combined with aluminum ion, 1507 to 1417 cm -1 It is the C=C vibration peak of benzene ring, 885 to 785 cm -1 It is the vibration peak after substitution of different conjugated rings, 463 cm -1 The vibration peak of Al-O indicates that the benzohydrazone aluminum complex has been succes...

Embodiment 2

[0031] The preparation method of benzohydrazone aluminum in this example is 0.3843g (1mmol) of N-((7-hydroxy-2-oxo-2H-benzopyran-3-one)yl-benzylidene)benzohydrazone Mix with 0.2281g (0.67mmol) of aluminum sulfate in 100mL of acetonitrile and reflux for 30 minutes, then add half of the solvent by rotary evaporation, then evaporate naturally to obtain light yellow crystals, wash with acetonitrile and ethanol several times, and dry in vacuum to obtain Product 0.40 g, yield 78%. from figure 2 It can be found that the complex synthesized in this example has two typical aluminum peaks, the first position is around 12ppm, and the other is around 69ppm. Around 12ppm is the classic carbonyl six-coordination structure, and around the second 69ppm is a four-coordination structure containing carbonyl and phenolic hydroxyl coordination. Therefore, it contains two different coordination modes, and contains two kinds of aluminum in the molecule, and the combination of the two kinds of alu...

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Abstract

The invention relates to a detection method of capsaicin, which uses a benzohydrazone aluminum complex having the structure shown in formula II as a fluorescent detection chromogenic agent for capsaicin. The capsaicin detection method of the invention is convenient and easy to implement, has high sensitivity and strong anti-interference ability, can quickly detect capsaicin, and can be used for detection of waste oil, food additives, environment and the like.

Description

technical field [0001] The invention relates to a method for detecting capsaicin by using fluorescent color development, and belongs to the technical field of detection. Background technique [0002] Waste oil generally refers to all kinds of low-quality oils that exist in people's lives, and its source is mainly the grease trap of the hotel sewer. Contains aflatoxin, polycyclic aromatic hydrocarbons, impurity metal ions and other impurities. Once "gutter oil" is ingested, white blood cells and the mucous membrane of the digestive tract are easily damaged, causing food poisoning and even cancer. The detection of waste oil is a complicated problem. The reason is that waste oil has complex components and low impurity content. It is similar to fake oil, inferior oil, and industrial vegetable oil. It is difficult to pass the identification of polycyclic aromatic hydrocarbons, impurity metal ions, and yellow yeast. Mycin was used to determine that it was waste oil. [0003] Rec...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439G01N2021/6432
Inventor 程如梅区升举文杨明葛春
Owner 杭州柘大飞秒检测技术有限公司
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