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Method for simultaneously detecting contents of four components of traditional Chinese medicine for treating children cough

A technology for traditional Chinese medicine and children, applied in the field of drug analysis, can solve problems such as single measurement index, and achieve the effects of high detection efficiency, large peak area and low cost

Active Publication Date: 2020-03-31
JIANMIN PHARMA GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for simultaneously detecting the content of four components of liquiritin, naringin, neohesperidin and arctiin in view of the single defect of the existing traditional Chinese medicine content determination index for treating children's cough

Method used

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  • Method for simultaneously detecting contents of four components of traditional Chinese medicine for treating children cough
  • Method for simultaneously detecting contents of four components of traditional Chinese medicine for treating children cough
  • Method for simultaneously detecting contents of four components of traditional Chinese medicine for treating children cough

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Embodiment 1

[0030] The selection of embodiment 1 mobile phase

[0031] Preparation of sample solution: take Xiaoer Xuanfei cough syrup, shake well, accurately measure 5ml, put it in a 100ml measuring bottle, dissolve it with 75% (volume) methanol and dilute to the mark, shake well, filter, take the subsequent filtrate, and get .

[0032] Instrument: Agilent1100 high performance liquid chromatography series (Agilent Technologies, USA, including quaternary pump, automatic sampler, column thermostat, ultraviolet detector, Chemstation chromatography workstation);

[0033] Mobile phase 1: Acetonitrile is mobile phase A, water is mobile phase B;

[0034] Mobile phase 2: acetonitrile is mobile phase A, and 0.2% (volume) phosphoric acid aqueous solution is mobile phase B;

[0035] Mobile phase 3: Acetonitrile containing 0.1% (volume) bromoethane was mobile phase A, and 0.2% phosphoric acid aqueous solution was mobile phase B.

[0036] Gradient elution conditions: see Table 1.

[0037] Column ...

Embodiment 2

[0042] The selection of embodiment 2 chromatographic columns

[0043] Preparation of sample solution: Shake this product well, accurately measure 5ml, put it in a 100ml measuring bottle, dissolve it with 75% methanol and dilute to the mark, shake well, filter, and take the filtrate to get ready.

[0044] Instrument: Agilent1100 high performance liquid chromatography series (Agilent Technologies, USA, including quaternary pump, automatic sampler, column thermostat, ultraviolet detector, Chemstation chromatography workstation);

[0045] Mobile phase: Acetonitrile containing 0.1% bromoethane was mobile phase A, and 0.2% phosphoric acid aqueous solution was mobile phase B.

[0046] Gradient elution conditions: see Table 1.

[0047] Column temperature: 30°C; detection wavelength: 280nm; flow rate: 1.0ml / min; injection volume: 10ul

[0048] Chromatographic column 1: Agilent XDB-C18 chromatographic column (250×4.6mm, 5um)

[0049] Column 2: XB-NH 2 (250×4.6mm,5um)

[0050] Colum...

Embodiment 3

[0054] The selection of embodiment 3 wavelength

[0055]Refer to the provisions of "Chinese Pharmacopoeia" 2015 tablets, Arctium Fructus, Fructus Aurantii, Xiaoer Jingfeng San and Fuzi Lizhong Wan, the detection wavelength of arctiin in Arctium Fructus is 280nm, and the detection wavelength of naringin and neohesperidin in Fructus Aurantii The detection wavelength of Liquiritin in Xiaoer Jingfeng San and Fuzi Lizhong Pills is 276nm. Based on the above literature, 280nm is finally selected as the detection wavelength of this method.

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Abstract

The invention discloses a method for simultaneously detecting the contents of four components of a traditional Chinese medicine for treating children cough. The traditional Chinese medicine for treating children cough is prepared from burdock, mulberry leaves, bulbus fritillariae cirrhosae, mint, radix isatidis, schizonepeta spike, fructus aurantii, radix peucedani, aster and liquorice, the four components are liquiritin, naringin, neohesperidin and arctiin, and the method comprises the following steps: extracting a sample to be detected with an extraction solvent, and detecting with a high performance liquid chromatograph. According to the method, the contents of the four substances can be detected at the same time, the adopted chromatographic conditions are good in separation effect, short in peak appearance time, good in detection result reproducibility and high in accuracy, and the quality of the traditional Chinese medicine is more controllable.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to a method for simultaneously detecting the contents of four components in traditional Chinese medicine for treating children's cough. Background technique [0002] CN 101607032A discloses a traditional Chinese medicine for treating children's cough, which is made of ten traditional Chinese medicinal materials of burdock fruit, mulberry leaves, fritillary fritillary, mint, isatid root, nepeta ear, aurantium aurantii, peucedanum, aster, and licorice. It is used for the treatment of exogenous cough in children caused by acute trachea-bronchitis. Currently, Jianmin Pharmaceutical Group Co., Ltd. is conducting new drug clinical research. It belongs to the six new types of traditional Chinese medicine. [0003] At present, the quality control of this traditional Chinese medicine is mainly through the detection of the content of arctiin, but the quantitative detection of a single component i...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/74
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N2030/027
Inventor 熊莉吴木琴任霞向阳王碧波熊桓杨颃卢敏陈鹏余丽花万哲王涛涛王丫头
Owner JIANMIN PHARMA GRP CO LTD
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