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A cell fusion strain and its application

A cell fusion and strain technology, applied in bacteria, applications, fungi, etc., can solve the problems of limited application of photosynthetic bacteria, high crude fiber content, low digestibility, etc., and achieve the effect of improving feed value

Active Publication Date: 2021-10-26
YIBIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high crude fiber content, low digestibility, and low protein content, the research on corn stalks is mainly focused on the production of biogas, compost, and returning to the field, and less than 10% of them are used as feed
However, photosynthetic bacteria themselves cannot utilize xylose, the second largest degradation product of corn stalks, which greatly limits the application of photosynthetic bacteria in transforming straw into feed. the meaning of

Method used

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  • A cell fusion strain and its application
  • A cell fusion strain and its application
  • A cell fusion strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1.1 Strains and raw materials

[0034] Strains: Rhodopseudomonas palustris and Candida shohata were purchased from China General Microorganism Culture Collection and Management Center, and the numbers were CGMCC NO.12349 and CGMCC NO.24317 respectively.

[0035] Raw materials: collect corn stalks harvested from corn seeds in Nanxi District, Yibin, Sichuan, crush them on the spot to 5-8 cm, dry them in a constant temperature blast drying oven at 85°C until the water content is below 10%, and then crush them with a multi-functional grinder for 40 Mesh sieve.

[0036] 1.2 Main instruments and reagents

[0037] F800 fiber tester; SOX500 fat tester, Haineng Instrument; Kjeltec 8400 Kjeldahl nitrogen analyzer, FOSS. Lysozyme, Helicase

[0038] 1.3 Main medium

[0039] Photosynthetic bacteria seed medium (g / L): NaHCO 3 1.0, KH 2 PO 4 1.0, Na 2 SO 3 0.1, MgSO 4 ·7H 2 O0.01, CaCl 2 0.0005, FeSO 4 ·7H 2 O 0.001, NH 4 Cl 1.0, yeast extract 1.0, peptone 1.0, sodi...

Embodiment 2

[0062] Determination of Xylose Utilization Ability of Fusogens

[0063] The bacterial strain preserved in Example 1 was inserted into 100mL culture solution containing xylose as the only carbon source by 1% inoculum size, and the xylose content change in the culture solution was measured after inoculation 0h, 24h, 48h, and 72h. The results are shown in Table 3

[0064] Table 3 The results of determination of xylose utilization ability of fusion son

[0065]

[0066] Note: RZZ is a fusion; YZZ is Rhodopseudomonas palustris; XHT is Candida shohata

[0067] It can be seen from Table 3 that the xylose concentration in the culture solution was measured 24 hours after the inoculation of the three strains, and the xylose utilization rates of Rhodopseudomonas palustris, Candida shohata and the fusion were 3.2% and 37.36% respectively. and 86.04%, and the utilization rates were 31%, 83.19% and 92% after 72 hours. Fusion RZZ's ability to utilize xylose was significantly improved, ...

Embodiment 3

[0069] Fermentation conversion of straw and process optimization

[0070] 1) Straw pretreatment. Weigh 100g of corn stalks in a 1L beaker and use 0.75% dilute sulfuric acid to adjust the solid-liquid ratio to 1:5, then transfer to a 1.5L stainless steel tubular reactor for acidification at 150°C for 1 hour to obtain acidified grains, and wait until the temperature and pressure drop Then open the cover, add 10mL ammonia water and treat at 100°C for 2 hours to get ammoniated grains, add 0.8% (v / v) ammonia water to adjust the pH to 5.0, add cellulase to 200U / g material, 50°C, 120rpm shaker hydrolysis for 72 hours .

[0071] 2) Preparation of starter. The bacterial strain preserved in Example 1 was then inoculated in the enzyme-treated tank with 80% inoculum amount by the three-stage cultivation process, and cultivated at 28° C. for 5 days, which was the first-stage cultivation. Inoculate 50% of the first-level culture solution in the enzyme-treated grains with the same culture...

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Abstract

The invention provides a cell fusion bacterial strain and its application, and relates to the technical field of cell fusion. The preservation number of the cell fusion strain of the present invention is CGMCC NO.19010. The utilization rate of 0.4% xylose was 86.04% 24 hours after the cell fusion strain was inoculated for 6 times, and the utilization ability of xylose was significantly higher than that of the two parental strains. The pretreated corn stalks were fermented and transformed by using the cell fusion strain at a material-to-water ratio of 1:9, a bad layer of 4 cm, a temperature of 29° C., a shaking table speed of 120 rpm, a fermentation time of 4.5 days, and an inoculum size of 8.5%. The fermented corn stalks True protein increased from 4.12% to 21.87%, crude fiber decreased from 37.53% to 16.37%, crude fat increased from 4.43% to 6.51%, crude ash decreased from 5.84% to 4.23%, and the moisture content of fermented dry grains was 9.59%. feed value of corn stalks.

Description

technical field [0001] The invention belongs to the technical field of cell fusion, and in particular relates to a cell fusion bacterial strain and its application. Background technique [0002] Photosynthetic bacteria (Photosynthetic Bacteria, referred to as PSB) is a kind of prokaryotic organisms that can carry out non-oxygenous photosynthesis. Swamps, rivers and seas, lakes, soil and paddy fields in nature. The bacterium is rich in protein (65%), soluble sugar (20%), and fat (7%), and also contains antiviral substances, growth-promoting factors, and physiologically active substances, and is non-toxic and has no side effects. At present, it has been widely used in the fields of organic wastewater treatment, biohydrogen production, biopharmaceuticals, food industry and animal breeding. In recent years, studies on the use of photosynthetic bacteria in the production of single-cell protein feed have been reported successively. Qiu Hongduan et al. used brewing sauce dregs to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12N1/19A23K10/18A23K10/12C12R1/72C12R1/01
CPCA23K10/12A23K10/18C12N1/165C12N1/205C12R2001/01C12R2001/72
Inventor 张健李国强康佳炜
Owner YIBIN UNIV